Structural relationships between diverse cis-acting elements are critical for the functional properties of a rbcS minimal light regulatory unit

Journal of Experimental Botany
Luisa López-OchoaLuis Herrera-Estrella

Abstract

Light-regulation of photosynthesis-associated nuclear genes is mediated by multipartite cis-regulatory units located in their promoter regions. The combination, spacing, and relative orientation of transcription factor binding sites in these units influences the assembly of specific multiprotein complexes that control gene expression. In this work, the functional architecture of the conserved modular array 5 (CMA5), a previously characterized minimal light-regulatory unit of rbcS gene promoters, has been analysed. With the aim of defining the sequences of CMA5 that, besides the I- and G-box elements, are essential for CMA5 responsiveness to light and chloroplast-derived signals, a series of mutations affecting the sequences flanking these elements was performed. It was found that an I-box associated module, named IbAM5, is essential for CMA5 functionality and is able to bind nuclear proteins in vitro. The spacing requirements of the I- and G-box elements in achieving adequate combinatorial interaction were also studied as well as the effect of interchanging the relative position of these elements in the native rbcS promoter arrangement. The results show that helical phasing and distance between the I- and G-box motifs are criti...Continue Reading

Citations

Jul 11, 2013·Photosynthesis Research·James O BerryChristopher M Mure
Jan 13, 2015·Journal of Genetics·Mojtaba RanjbarHoshang Alizadeh
Jun 30, 2016·Scientific Reports·Frédéric BouchéClaire Périlleux
Jul 24, 2015·Cellular & Molecular Biology Letters·Manoj Kumar SekhwalRenu Sarin
Nov 17, 2010·Molecular Biology Reports·Youru WangShiyun Chen
Jul 21, 2021·BMC Genomics·Adriana M GallegoNatalia Pabón-Mora

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