PMID: 9182660Jun 16, 1997Paper

Structural requirements for basolateral sorting of the human transferrin receptor in the biosynthetic and endocytic pathways of Madin-Darby canine kidney cells

The Journal of Cell Biology
G Odorizzi, I S Trowbridge

Abstract

In polarized Madin-Darby canine kidney (MDCK) cells, the transferrin receptor (TR) is selectively delivered to the basolateral surface, where it internalizes transferrin via clathrin-coated pits and recycles back to the basolateral border. Mutant tailless receptors are sorted randomly in both the biosynthetic and endocytic pathways, indicating that the basolateral sorting of TR is dependent upon a signal located within the 61-amino acid cytoplasmic domain. To identify the basolateral sorting signal of TR, we have analyzed a series of mutant human TR expressed in MDCK cells. We find that residues 19-41 are sufficient for basolateral sorting from both the biosynthetic and endocytic pathways and that this is the only region of the TR cytoplasmic tail containing basolateral sorting information. The basolateral sorting signal is distinct from the YTRF internalization signal contained within this region and is not tyrosine based. Detailed functional analyses of the mutant TR indicate that residues 29-35 are the most important for basolateral sorting from the biosynthetic pathway. The structural requirements for basolateral sorting of internalized receptors from the endocytic pathway are not identical. The most striking difference is ...Continue Reading

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Citations

Sep 3, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R HeilkerP Crottet
Dec 13, 2006·Journal of Molecular Evolution·Lisa Ann Lambert, Stacey L Mitchell
Feb 24, 2001·Molecular Aspects of Medicine·P T LieuY Yang
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May 19, 2006·Molecular Biology of the Cell·Viktor JakobAnna Starzinski-Powitz
Sep 21, 2007·Molecular Biology of the Cell·Jorge CancinoAlfonso González
Nov 14, 2008·Molecular Biology of the Cell·Maribel DonosoMaría-Paz Marzolo
Mar 17, 2004·Current Opinion in Pediatrics·Julie A Nicoletta, George J Schwartz
Oct 31, 2008·American Journal of Physiology. Renal Physiology·Olga VaginGeorge Sachs
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Jan 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Diego GravottaEnrique Rodriguez-Boulan
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Jan 8, 2014·The Journal of Cell Biology·Juan S Bonifacino
Oct 27, 2009·FEBS Letters·Alfonso Gonzalez, Enrique Rodriguez-Boulan

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