Structural requirements for bone sialoprotein binding and modulation of matrix metalloproteinase-2.

Biochemistry
Alka JainNeal S Fedarko

Abstract

Bone sialoprotein (BSP) has been shown to induce limited gelatinase activity in latent matrix metalloproteinase-2 (MMP-2) without removal of the propeptide and to restore enzymatic activity to MMP-2 previously inhibited by tissue inhibitor of matrix metalloproteinase-2 (TIMP2). The current study identifies structural domains in human BSP and MMP-2 that contribute to these interactions. The 26 amino acid domain encoded by exon 4 of BSP is shown by a series of binding and activity assays to be involved in the displacement of MMP-2's propeptide from the active site and thereby inducing the protease activity. Binding assays in conjunction with enzyme activity assays demonstrate that both amino- and carboxy-terminal domains of BSP contribute to restoration of activity to TIMP2-inhibited MMP-2, while the MMP-2 hemopexin domain is not required for reactivation.

References

Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H E Van Wart, H Birkedal-Hansen
Aug 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K P MintzL W Fisher
Jan 1, 1996·Enzyme & Protein·M L CorcoranW G Stetler-Stevenson
Mar 1, 1997·Matrix Biology : Journal of the International Society for Matrix Biology·G Murphy, V Knäuper
Aug 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J T StubbsL W Fisher
Feb 13, 2001·Biochemical and Biophysical Research Communications·L W FisherN S Fedarko
Nov 1, 2001·Biochimica Et Biophysica Acta·A N Webber, W Lubitz
Feb 5, 2002·Biochimica Et Biophysica Acta·G F Weber
Feb 10, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Neal S FedarkoLarry W Fisher
Jun 17, 2004·Journal of the National Cancer Institute·Abdullah KaradagLarry W Fisher
Dec 30, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Larry W FisherNeal S Fedarko
Aug 2, 2005·Trends in Biochemical Sciences·Peter TompaLászló Buday
Nov 23, 2006·Cytokine & Growth Factor Reviews·Mohamed K El-TananiPhilip S Rudland
Jan 11, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Kalu U E Ogbureke, Larry W Fisher
Feb 6, 2007·Bioorganic & Medicinal Chemistry·Rajeshwar P Verma, Corwin Hansch
May 15, 2007·The Journal of Biological Chemistry·Brian ChristensenEsben S Sørensen
Feb 23, 2008·Nature Reviews. Cancer·Akeila BellahcèneNeal S Fedarko

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Citations

Nov 3, 2010·The Journal of Biological Chemistry·Bon-Hun KooDoo-Sik Kim
Oct 19, 2011·Journal of Cellular Biochemistry·Stéphane BarthelemiSandrine Lorimier
Nov 23, 2010·The FEBS Journal·Elin Hadler-OlsenJan-Olof Winberg
Mar 14, 2013·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ann Iren SolliElin Hadler-Olsen

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