Structural snapshots of human pre-60S ribosomal particles before and after nuclear export.

Nature Communications
Xiaomeng LiangNing Gao

Abstract

Ribosome biogenesis is an elaborate and energetically expensive program that involve two hundred protein factors in eukaryotes. Nuclear export of pre-ribosomal particles is one central step which also serves as an internal structural checkpoint to ensure the proper completion of nuclear assembly events. Here we present four structures of human pre-60S particles isolated through a nuclear export factor NMD3, representing assembly stages immediately before and after nuclear export. These structures reveal locations of a dozen of human factors, including an uncharacterized factor TMA16 localized between the 5S RNA and the P0 stalk. Comparison of these structures shows a progressive maturation for the functional regions, such as peptidyl transferase centre and peptide exit tunnel, and illustrate a sequence of factor-assisted rRNA maturation events. These data facilitate our understanding of the global conservation of ribosome assembly in eukaryotes and species-specific features of human assembly factors.

References

May 30, 2003·The EMBO Journal·Christopher R TrottaJames E Dahlberg
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Sep 27, 2005·Journal of Structural Biology·David N Mastronarde
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Mar 3, 2010·Blood·Anupama Narla, Benjamin L Ebert
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Aug 2, 2011·Blood·Chi C WongAlan J Warren
Sep 25, 2012·Journal of Structural Biology·Sjors H W Scheres
Oct 10, 2012·Molecular and Cellular Biology·Jochen BasslerHelmut Bergler
Nov 13, 2012·Nature Structural & Molecular Biology·Bettina BradatschEd Hurt
Nov 13, 2012·Nature Structural & Molecular Biology·Basil J GreberNenad Ban
Feb 5, 2013·Trends in Cell Biology·Katrin Karbstein
Nov 6, 2013·Genetics·John L Woolford, Susan J Baserga
Nov 12, 2013·Nature Methods·Alp KucukelbirHemant D Tagare
Mar 29, 2014·Science·Alexey AmuntsV Ramakrishnan
Oct 28, 2014·Wiley Interdisciplinary Reviews. RNA·Anthony K HenrasPierre-Emmanuel Gleizes
Jan 13, 2015·Blood·Kim De KeersmaeckerJonathan D Dinman
Apr 17, 2015·Genes & Development·Kathleen L McCannSusan J Baserga
May 11, 2015·Cell·Elmar BehrmannChristian M T Spahn
Jul 30, 2015·Nature·Ningning LiNing Gao
Aug 12, 2015·Proceedings of the National Academy of Sciences of the United States of America·Taiga AraiTsutomu Suzuki
Nov 26, 2015·Journal of Structural Biology·Kai Zhang
Aug 18, 2016·Nucleic Acids Research·Natacha LarburuCélia Plisson-Chastang
Nov 1, 2016·Nature Structural & Molecular Biology·Anshuk SarkarEd Hurt
Jan 24, 2017·Nature Structural & Molecular Biology·Chengying MaNing Gao
Apr 25, 2017·Nature Reviews. Cancer·Morgan L Truitt, Davide Ruggero
Jun 6, 2017·Trends in Biochemical Sciences·Dieter KresslerJochen Baßler
Jul 16, 2017·Protein Science : a Publication of the Protein Society·Thomas D GoddardThomas E Ferrin
Sep 8, 2017·Nature Structural & Molecular Biology·Cohue PeñaVikram Govind Panse
Oct 27, 2017·Protein Science : a Publication of the Protein Society·Christopher J WilliamsDavid C Richardson
Nov 4, 2017·Science·Eric W Mills, Rachel Green
Nov 17, 2017·Nature·S Kundhavai NatchiarBruno P Klaholz
Dec 2, 2017·Nature Reviews. Cancer·Joffrey PelletierSiniša Volarević

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Citations

Oct 26, 2020·Current Opinion in Structural Biology·Meredith N FrazierRobin E Stanley
Nov 25, 2020·Cells·Daniela Pollutri, Marianna Penzo
Jan 23, 2021·RNA·Varun BhaskarJeffrey A Chao
Oct 29, 2021·Nature Communications·Jan Andreas RulandUlrich Kubitscheck

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Methods Mentioned

BETA
affinity purification
GTPase
PCR
transfection
NMR

Software Mentioned

Molprobity
PSIPRED
RELION
BLAST
pFind3
MotionCorr2
CryoID
SPIDER
pLink2
PHENIX

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