Structural studies of heparan sulfate hexasaccharides: new insights into iduronate conformational behavior

Journal of the American Chemical Society
Kevin J MurphyDavid A Pye

Abstract

There is a growing opinion that the conformational dynamics within HS chains is critical to their observed biological activities. Investigations into HS conformational dynamics are problematic, given the structural complexity and heterogeneity of HS chains. However, this goal will be more obtainable once we understand the important roles HS sequence/sulfation patterns play in determining the conformational dynamics of iduronate units. This is the first study to compare isomers of N-sulfated oligosaccharides, with respect to the conformational versatility of their internal iduronates. Characterization by NMR spectroscopy of two HS oligosaccharides derived from porcine mucosal HS enabled the measurement of iduronate coupling constants, while under the influence of different flanking saccharide sequences. By fitting our coupling constant data to a new set of theoretical coupling constants, calculated using explicit water molecular dynamic simulations, we are able to offer new insights into the role sequence/sulfation patterns play in influencing iduronate conformational behavior. Fitting of experimental data, using our new theoretically derived coupling constants, suggests that replacement of the N-sulfate group to the reducing si...Continue Reading

Citations

Aug 21, 2012·The Journal of Organic Chemistry·Steen U HansenJohn M Gardiner
Mar 26, 2010·Physical Chemistry Chemical Physics : PCCP·Marion JasninGiuseppe Zaccai
Aug 1, 2009·Glycobiology·Neha S Gandhi, Ricardo L Mancera
Feb 25, 2014·Progress in Biophysics and Molecular Biology·Vitor H Pomin
Sep 14, 2010·Current Opinion in Structural Biology·T R RuddE A Yates
Oct 15, 2011·Angewandte Chemie·Philippe OchsenbeinMohamed El-Hajji
Feb 27, 2020·Nature Reviews. Neurology·Susan L LindsaySusan C Barnett
May 14, 2019·Wiley Interdisciplinary Reviews. Computational Molecular Science·Balaji NagarajanUmesh R Desai
Jun 3, 2014·The Analyst·Vitor H Pomin
Jul 3, 2019·Biology·George A McCanneySusan C Barnett

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