Structural studies of hydrated samples of amorphous calcium phosphate and phosphoprotein nanoclusters

European Biophysics Journal : EBJ
Samuel LentonSusana C M Teixeira

Abstract

There are abundant examples of nanoclusters and inorganic microcrystals in biology. Their study under physiologically relevant conditions remains challenging due to their heterogeneity, instability, and the requirements of sample preparation. Advantages of using neutron diffraction and contrast matching to characterize biomaterials are highlighted in this article. We have applied these and complementary techniques to search for nanocrystals within clusters of calcium phosphate sequestered by bovine phosphopeptides, derived from osteopontin or casein. The neutron diffraction patterns show broad features that could be consistent with hexagonal hydroxyapatite crystallites smaller than 18.9 Å. Such nanocrystallites are, however, undetected by the complementary X-ray and FTIR data, collected on the same samples. The absence of a distinct diffraction pattern from the nanoclusters supports the generally accepted amorphous calcium phosphate structure of the mineral core.

References

Mar 1, 1984·Calcified Tissue International·D G Nelson, J D McLean
Sep 28, 1984·Biochimica Et Biophysica Acta·R L LysterR J Williams
Nov 8, 1983·Biochimica Et Biophysica Acta·T C McGannT Richardson
Jan 1, 1982·Calcified Tissue International·F R Bringhurst, J T Potts
Nov 24, 1982·Biochimica Et Biophysica Acta·C HoltD W Hukins
Jul 1, 1996·The Biochemical Journal·G K HunterH A Goldberg
Dec 22, 1999·Progress in Biophysics and Molecular Biology·J L Arrondo, F M Goñi
Sep 25, 2003·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·UNKNOWN Adele Boskey
Oct 8, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Patrick S StaytonMichele Gilbert
May 4, 2004·Micron : the International Research and Review Journal for Microscopy·R F EgertonM Malac
Jan 20, 2005·The Journal of Biological Chemistry·Keith J CrossEric C Reynolds
Jul 12, 2005·Calcified Tissue International·A GerickeA L Boskey
Feb 21, 2006·Journal of Biomechanics·Jeffry S NymanXiaodu Wang
Nov 13, 2007·Journal of the American Chemical Society·Bernd GroheGraeme K Hunter
Mar 19, 2009·The FEBS Journal·Carl HoltRoger A Clegg
Nov 10, 2009·Journal of the American Chemical Society·Peizhi ZhuAyyalusamy Ramamoorthy
Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Julia MahamidLia Addadi
Aug 5, 2010·Journal of the American Chemical Society·Jiadi XuAyyalusamy Ramamoorthy
Feb 23, 2011·The Journal of Physical Chemistry. B·Ronald GebhardtWolfgang Doster
Sep 8, 2012·The Journal of Physical Chemistry. B·Kamal H MroueAyyalusamy Ramamoorthy
Nov 22, 2013·Nature Materials·Melinda Duer, Arthur Veis
Nov 26, 2013·Journal of Solid State Chemistry·John-David P McElderryMichael D Morris
Dec 10, 2013·Journal of Structural Biology·Carl HoltSusana C M Teixeira
Jan 29, 2014·Nature Communications·Johannes IhliFiona C Meldrum
May 13, 2014·Acta Crystallographica. Section A, Foundations and Advances·Paul F Fewster
Jan 1, 2012·Faraday Discussions·Fabio NudelmanNico A J M Sommerdijk
Jan 1, 2003·Journal of Research of the National Institute of Standards and Technology·D SkrticE D Eanes

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