Structural studies of the periplasmic portion of the diguanylate cyclase CdgH from Vibrio cholerae

Scientific Reports
Min XuWei Xie

Abstract

Cyclic diguanylate monophosphate (c-di-GMP) is a second messenger involved in bacterial signal transduction and produced by diguanylate cyclases (DGCs) generally containing highly variable periplasmic signal-recognition domains. CdgH is a DGC enzyme that regulates rugosity associated phenotypes in Vibrio cholerae. CdgH has two N-terminal tandem periplasmic substrate-binding (PBPb) domains for its signal recognition; however, the role of the tandem PBPb domains remains unclear. Here, we reported the crystal structure of the periplasmic portion of CdgH, which indicated that both tandem PBPb domains consist of typical interlobe ligand-binding architecture. Unexpectedly, the PBPb-I domain binds an L-arginine which apparently has been co-purified from the E. coli expression system, whereas the PBPb-II domain is in an unliganded open state. Structural comparison with other amino acid-binding proteins indicated that despite similar ligand-binding pockets, the PBPb-I domain possesses two ligand-binding residues (E122 and Y148) not conserved in homologs and involved in hydrophilic and hydrophobic interactions with L-arginine. Isothermal titration calorimetry indicated that the PBPb-I is primarily an L-arginine/L-lysine/L-ornithine-bindi...Continue Reading

References

Jun 1, 1990·Applied and Environmental Microbiology·M L TamplinR R Colwell
Jul 17, 2004·Molecular Microbiology·Anna D Tischler, Andrew Camilli
Nov 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Carsten MatzStaffan Kjelleberg
Apr 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Shah M FaruqueJohn J Mekalanos
Aug 10, 2006·Annual Review of Genetics·Urs Jenal, Jacob Malone
Oct 10, 2006·Journal of Bacteriology·Robert P RyanJ Maxwell Dow
Jul 21, 2007·The Journal of Biological Chemistry·Ralf PaulUrs Jenal
Oct 31, 2007·Proceedings of the National Academy of Sciences of the United States of America·Munirul AlamRita R Colwell
Sep 17, 2009·Nature Reviews. Microbiology·Tilman Schirmer, Urs Jenal
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Jul 30, 2011·Current Opinion in Infectious Diseases·Richelle C Charles, Edward T Ryan
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
Jan 10, 2012·Current Opinion in Microbiology·Holger SondermannFitnat H Yildiz
Apr 17, 2012·Acta Crystallographica. Section D, Biological Crystallography·Pavel V AfoninePaul D Adams
Mar 9, 2013·Microbiology and Molecular Biology Reviews : MMBR·Ute RömlingMark Gomelsky
Apr 23, 2014·Nucleic Acids Research·Xavier Robert, Patrice Gouet
May 7, 2014·Infection and Immunity·Benjamin J Koestler, Christopher M Waters
Apr 8, 2015·Biochemical and Biophysical Research Communications·Xuan YangTao Jiang
Apr 22, 2015·Nature Reviews. Microbiology·Jennifer K TeschlerFitnat H Yildiz
Apr 30, 2016·The Journal of Biological Chemistry·Martina Valentini, Alain Filloux
Aug 9, 2016·Journal of Molecular Biology·Tilman Schirmer
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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Citations

Feb 6, 2018·FEMS Microbiology Letters·Serena RinaldoFrancesca Cutruzzolà
Nov 17, 2020·Frontiers in Cellular and Infection Microbiology·Sumit BiswasDivya Bandekar

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Methods Mentioned

BETA
isothermal titration calorimetry

Software Mentioned

PHENIX
Sim
DALI
Arnt
COOT
HKL2000
OriginLab
PyMOL
Per
SEDFIT

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