PMID: 702639Sep 1, 1978Paper

Structural studies on Rauscher murine leukemia virus: isolation and characterization of viral envelopes

Journal of Virology
W J van de VenH Bloemendal

Abstract

A preparative method for isolating pure viral envelopes from a type-C RNA tumor virus, Rauscher murine leukemia virus, is described. Fractionation of virions of Rauscher murine leukemia virus was studied after disruption of the virions with the detergents sodium dodecyl sulfate of Nonidet P-40 in combination with ether. Fractionation was performed through flotation in a discontinuous sucrose gradient and, as appeared from electron microscopic examination, a pure viral envelope fraction was obtained in this way. By use of sensitive competition radioimmunoassays or sodium dodecyl sulfate-polyacrylamide gel electrophoresis after immunoprecipitation with polyvalent and monospecific antisera directed against Rauscher murine leukemia virus proteins, the amount of the gag and env gene-encoded structural polypeptides in the virions and the isolated envelope fraction was compared. The predominant viral structural polypeptides in the purified envelope fraction were the env gene-encoded polypeptides gp70, p15(E), and p12(E), whereas, except for p15, there was only a relatively small amount of the gag gene-encoded structural polypeptides in this fraction.

References

Aug 12, 1976·Nature·M BarbacidS A Aaronson
Jan 13, 1978·Science·D P BolognesiW Schäfer
Sep 1, 1977·Journal of Virology·W L KarshinR B Arlinghaus
Nov 1, 1971·Virology·R B Luftig, S S Kilham
Aug 1, 1970·Virology·P H DuesbergP K Vogt
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Jan 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·P H Duesberg, W S Robinson
Jul 1, 1965·The Journal of Cell Biology·M G Farquhar, G E Palade
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER

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Citations

Jan 1, 1977·Medical Microbiology and Immunology·D P BolognesiS J Sullivan
Nov 1, 1979·Journal of Virology·S J AndersonJ M Bowen
Oct 30, 1983·Virology·C A Edbauer, R B Naso
Jul 15, 1979·Journal of Molecular Biology·R B Pepinsky, V M Vogt

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