Structural study of a highly O-acetylated core of Legionella pneumophila serogroup 1 lipopolysaccharide

Carbohydrate Research
Y A KnirelU Zähringer

Abstract

A core oligosaccharide was obtained after mild acid degradation of Legionella pneumophila serogroup 1 lipopolysaccharide (LPS). On the basis of chemical, GLC-MS, 1H, and 13C NMR spectroscopic data, it was found that the oligosaccharide obtained is a highly O-acetylated heptasaccharide having the following structure: [formula: see text] where Kdo is 3-deoxy-D-manno-octulosonic acid and QuiNAc is 2-acetamido-2,6-dideoxyglucose. In the LPS, the O-specific polysaccharide chain is linked to position 3 of the terminal rhamnosyl group and is cleaved during degradation of the LPS. The degradation also induced partial migration and partial removal of the O-acetyl group from the terminal rhamnosyl group which, together with the occurrence of the reducing Kdo residue in multiple forms, contributes to the heterogeneity of the isolated core oligosaccharide. No such highly O-acetylated core oligosaccharide has been reported so far for LPS of Gram-negative bacteria.

References

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Citations

Jul 7, 1998·The Journal of Experimental Medicine·E LünebergM Frosch
Mar 23, 2018·FEMS Microbiology Letters·Helen Y BuseDarren A Lytle
May 13, 2014·The Journal of Biological Chemistry·Tiezheng LiK Dale Noel
Feb 23, 2007·Journal of Medical Microbiology·Etsu T FuseKeizo Yamaguchi
Jan 11, 2020·Frontiers in Microbiology·Marta Palusinska-SzyszBozena Kowalczyk
Jun 28, 2020·Cellular and Molecular Life Sciences : CMLS·Diego García-Weber, Cécile Arrieumerlou
Feb 6, 2021·International Journal of Molecular Sciences·Bozena KowalczykMarta Palusinska-Szysz

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