Structural Switch from Hairpin to Duplex/Antiparallel G-Quadruplex at Single-Nucleotide Polymorphism (SNP) Site of Human Apolipoprotein E (APOE ) Gene Coding Region

ACS Omega
Swati ChaudharyShrikant Kukreti

Abstract

A gradual dementia, which leads to the loss of memory and intellectual abilities, is the main characteristics of Alzheimer's disease. Amyloid-β (Aβ) plaques are the main components that accumulate and form clumps in the brains of people suffering from Alzheimer's disease. Apolipoprotein E (APOE), an amyloid-binding protein is considered as one of the main genetic risk factor of the late-onset Alzheimer's disease. Different isoforms of APOE gene named APOE2, APOE3, and APOE4 are known to exist, which differ from each other at certain positions involving single-nucleotide polymorphisms (SNPs). Out of these isoforms, APOE4 increases the risk of developing late-onset Alzheimer's disease, whereas APOE3 is the most common among the general population. APOE4 differs from the common APOE3 by only one nucleotide at position +2985 (T to C), which results in immense alteration in the structure and function of the APOE gene. A combination of gel electrophoresis (polyacrylamide gel electrophoresis, PAGE), circular dichroism (CD), CD melting, thermal difference spectra and UV-thermal denaturation (TM) techniques was used to investigate the structural polymorphism associated with T → C single-nucleotide polymorphism (SNP) at the GC-rich seque...Continue Reading

References

Jan 1, 1995·Annual Review of Biophysics and Biomolecular Structure·G Varani
Mar 18, 2004·Oligonucleotides·Jean-Louis Mergny, Laurent Lacroix
Feb 16, 2005·Sub-cellular Biochemistry·Donald B Carter
Sep 15, 2005·Nucleic Acids Research·Jean-Louis MergnyJonathan B Chaires
Dec 28, 2006·Neurochemical Research·Laura Canevari, John B Clark
Jul 31, 2007·Biophysical Journal·Srinivasaraghavan Kannan, Martin Zacharias
Sep 20, 2008·Nature Reviews. Neuroscience·Lars Bertram, Rudolph E Tanzi
Mar 11, 2009·Journal of the American Chemical Society·Kah Wai LimAnh Tuân Phan
Jul 28, 2010·Nucleic Acids Research·Aurore GuédinJean-Louis Mergny
Jun 15, 2012·Chirality·Michaela VorlíčkováJaroslav Kypr
Nov 20, 2012·Neurobiology of Aging·Andrew B WolfJon Valla
Jan 9, 2013·Nature Reviews. Neurology·Chia-Chen LiuGuojun Bu
Jan 9, 2014·Biochemical Pharmacology·John E Morley, Susan A Farr
Feb 25, 2014·Neuron·Takahisa KanekiyoGuojun Bu
Jul 6, 2014·Journal of Computer-aided Molecular Design·Pavan Kumar PingaliDhananjay Bhattacharyya
Apr 2, 2016·Oxidative Medicine and Cellular Longevity·Sandeep Kumar SinghGeorge Perry
Jan 28, 2016·Biochemistry and Biophysics Reports·Mahima KaushikShrikant Kukreti

❮ Previous
Next ❯

Citations

Sep 7, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Imee M A Del MundoGuliang Wang
Apr 28, 2021·The FEBS Journal·John W Cave, Dianna E Willis
May 19, 2021·Proceedings of the National Academy of Sciences of the United States of America·Jia-Yuan GongZheng Tan

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
circular dichroism

Related Concepts

Related Feeds

Alzheimer's Disease: APOE

Apolipoprotein E (APOE) polymorphic alleles are major genetic risk factors for Alzheimer's disease. Discover the latest research on APOE and other genetic determinants of Alzheimer's disease here.

Alzheimer's Disease: Genetics

Alzheimer's disease is a neurodegenerative disease. Discover genetic and epigenetic aspects of Alzheimer’s disease, including genetic markers and genomic structural variations with this feed.

ApoE Phenotypes

Apolipoprotein E (APOE) is a protein involved in fat metabolism and associated with the pathogenesis of Alzheimer's disease and cardiovascular disease. Here is the latest research on APOE phenotypes.

Alzheimer's Disease: APP

Amyloid precursor protein (APP) proteolysis is critical for the development of Alzheimer's disease, a neurodegenerative disease associated with accumulation of amyloid plaques in the brain. Here is the latest research on APP and Alzheimer's disease.

Alzheimer's Disease: Amyloid Beta

Alzheimer's disease is a neurodegenerative disease associated with the accumulation of amyloid plaques in the brain; these plaques are comprised of amyloid beta deposits. Here is the latest research in this field.