Structural transients of contractile proteins upon sudden ATP liberation in skeletal muscle fibers

Biophysical Journal
Jun'ichi WakayamaH Iwamoto

Abstract

Structural changes of contractile proteins were examined by millisecond time-resolved two-dimensional x-ray diffraction recordings during relaxation of skinned skeletal muscle fibers from rigor after caged ATP photolysis. It is known that the initial dissociation of the rigor actomyosin complex is followed by a period of transient active contraction, which is markedly prolonged in the presence of ADP by a mechanism yet to be clarified. Both single-headed (overstretched muscle fibers with exogenous myosin subfragment-1) and two-headed (fibers with full filament overlap) preparations were used. Analyses of various actin-based layer line reflections from both specimens showed the following: 1), The dissociation of the rigor actomyosin complex was fast and only modestly decelerated by ADP and occurred in a single exponential manner without passing through any detectable transitory state. Its ADP sensitivity was greater in the two-headed preparation but fell short of explaining the large ADP effect on the transient active contraction. 2), The decay of the activated state of the thin filament followed the time course of tension more closely in an ADP-dependent manner. These results suggest that the interplay between the reattached ac...Continue Reading

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Citations

May 3, 2012·Biochemistry·Yoshiteru YamadaNaoto Yagi
Feb 26, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Julien OchalaLars Larsson
Apr 24, 2008·Cell Motility and the Cytoskeleton·Hiroyuki Iwamoto
Oct 10, 2006·Biophysical Journal·Naoto Yagi
Apr 17, 2007·Journal of Molecular Biology·Hiroyuki IwamotoTetsuro Fujisawa
Jul 11, 2008·Journal of Muscle Research and Cell Motility·Hiroyuki Iwamoto
Nov 19, 2008·Physical Chemistry Chemical Physics : PCCP·Alexey V CherepanovHuub J M De Groot
Jun 15, 2018·International Journal of Molecular Sciences·Hiroyuki Iwamoto

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