Structural transitions in the scaffolding and coat proteins of P22 virus during assembly and disassembly

Biochemistry
R TumaG J Thomas

Abstract

An in vitro system for investigating the assembly of the Salmonella phage P22 has been exploited to elucidate the structural basis of recognition between scaffolding protein (gp8) and coat protein (gp5) subunits of the viral procapsid. Raman spectroscopy and circular dichroism have been employed to examine structural thermostabilities of both gp8 and gp5 in native procapsids, and to characterize structural changes accompanying scaffolding exit, procapsid expansion, and shell disassembly. It is found that the secondary structure of the isolated gp8 subunit is rich in alpha-helix (approximately 40%), is highly thermolabile, and is characterized by noncooperative unfolding (Tm approximately 49 degrees C). Conversely, the procapsid-bound gp8 subunit exhibits stabilization of its alpha-helical secondary structure, characterized by cooperative unfolding. Because cooperative unfolding of gp8 coincides with exit from the procapsid, the present results suggest that unfolding and release are coupled processes. Structural differences between procapsid-free and procapsid-bound gp8 subunits are also apparent in Raman markers which monitor environments of tyrosine and tryptophan side chains. Temperature-resolved Raman spectroscopy of the emp...Continue Reading

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Citations

Mar 26, 1999·Journal of Virological Methods·S KaminakaT Toyoda
Aug 11, 2012·The Journal of Biological Chemistry·G Pauline Padilla-MeierSherwood R Casjens
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·G J Thomas
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·R TumaG J Thomas
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Apr 2, 2008·Journal of Molecular Biology·Siew Lay PohPaulo Tavares
Apr 22, 2005·Journal of Molecular Biology·Peter R WeigeleSherwood R Casjens
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May 29, 1999·The Journal of Biological Chemistry·B Greene, J King
Feb 21, 2006·Physical Review Letters·Eldon Emberly, Ned S Wingreen
Dec 12, 2019·Nanoscale·Jhanvi Sharma, Trevor Douglas
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