Structural transitions of transmembrane helix 6 in the formation of metarhodopsin I.

The Journal of Physical Chemistry. B
Markus EilersSteven O Smith

Abstract

Absorption of light by the visual pigment rhodopsin triggers a rapid cis-trans photoisomerization of its retinal chromophore and a series of conformational changes in both the retinal and protein. The largest structural change is an outward tilt of transmembrane helix H6 that increases the separation of the intracellular ends of H6 and H3 and opens up the G-protein binding site. In the dark state of rhodopsin, Glu247 at the intracellular end of H6 forms a salt bridge with Arg135 on H3 to tether H6 in an inactive conformation. The Arg135-Glu247 interaction is broken in the active state of the receptor, and Arg135 is then stabilized by interactions with Tyr223, Met257, and Tyr306 on helices H5, H6, and H7, respectively. To address the mechanism of H6 motion, solid-state NMR measurements are undertaken of Metarhodopsin I (Meta I), the intermediate preceding the active Metarhodopsin II (Meta II) state of the receptor. (13)C NMR dipolar recoupling measurements reveal an interhelical contact of (13)Cζ-Arg135 with (13)Cε-Met257 in Meta I but not with (13)Cζ-Tyr223 or (13)Cζ-Tyr306. These observations suggest that helix H6 has rotated in the formation of Meta I but that structural changes involving helices H5 and H7 have not yet occurr...Continue Reading

References

Jun 13, 1978·Biochemistry·A G DoukasT G Ebrey
Jan 1, 1981·Vision Research·V L RatnerE E Fesenko
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·M EilersS O Smith
Jan 5, 2000·Journal of Magnetic Resonance·B M FungK Ermolaev
Aug 5, 2000·Science·K PalczewskiM Miyano
Jan 10, 2002·Journal of the American Chemical Society·Scott E FellerAlexander D MacKerell
Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tetsuji OkadaYoshinori Shichida
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Philip J ReevesH Gobind Khorana
Jul 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elsa C Y YanRichard A Mathies
Nov 1, 1963·The Journal of General Physiology·R G MATTHEWSG WALD
Aug 27, 2004·The EMBO Journal·Jonathan J RuprechtGebhard F X Schertler
Mar 17, 2005·Journal of Molecular Biology·Ashish B PatelSteven O Smith
Sep 20, 2005·Journal of Molecular Biology·Steffen LüdekeReiner Vogel
Oct 14, 2005·Journal of Computational Chemistry·James C PhillipsKlaus Schulten
Mar 24, 2006·Photochemistry and Photobiology·Jacqueline EppsDavid S Kliger
Apr 6, 2006·Angewandte Chemie·Hitoshi Nakamichi, Tetsuji Okada
Apr 26, 2006·Biochemistry·Ana Karin KusnetzowWayne L Hubbell
Jun 8, 2006·Annual Review of Biochemistry·Krzysztof Palczewski
Jul 26, 2006·The Journal of Physical Chemistry. B·Jeffery B KlaudaRichard W Pastor
Aug 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hitoshi Nakamichi, Tetsuji Okada

❮ Previous
Next ❯

Citations

Feb 16, 2016·Progress in Lipid Research·Tomohiro KimuraRichard M Epand
Feb 11, 2015·Biochimica Et Biophysica Acta·Ashley N MartfeldRoger E Koeppe
Jun 3, 2021·Biomolecules·Matthias Elgeti, Wayne L Hubbell
Dec 25, 2013·Chemical Reviews·Oliver P ErnstHideki Kandori
Aug 3, 2013·Chemical Reviews·Philip D KiserKrzysztof Palczewski

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Xavier DeupiJorg Standfuss
Current Opinion in Structural Biology
Guillaume LebonChristopher G Tate
© 2021 Meta ULC. All rights reserved