Structural variability in wild-type and bchQ bchR mutant chlorosomes of the green sulfur bacterium Chlorobaculum tepidum

Biochemistry
Swapna GanapathyHuub J M de Groot

Abstract

The self-aggregated state of bacteriochlorophyll (BChl) c molecules in chlorosomes belonging to a bchQ bchR mutant of the green sulfur bacteria Chlorobaculum tepidum, which mostly produces a single 17(2)-farnesyl-(R)-[8-ethyl,12-methyl]BChl c homologue, was characterized by solid-state nuclear magnetic resonance (NMR) spectroscopy and high-resolution electron microscopy. A nearly complete (1)H and (13)C chemical shift assignment was obtained from well-resolved homonuclear (13)C-(13)C and heteronuclear (1)H-(13)C NMR data sets collected from (13)C-enriched chlorosome preparations. Pronounced doubling (1:1) of specific (13)C and (1)H resonances revealed the presence of two distinct and nonequivalent BChl c components, attributed to all syn- and all anti-coordinated parallel stacks, depending on the rotation of the macrocycle with respect to the 3(1)-methyl group. Steric hindrance from the 20-methyl functionality induces structural differences between the syn and anti forms. A weak but significant and reproducible reflection at 1/0.69 nm(-1) in the direction perpendicular to the curvature of cylindrical segments observed with electron microscopy also suggests parallel stacking of BChl c molecules, though the observed lamellar spac...Continue Reading

References

Oct 8, 1998·Journal of Magnetic Resonance·M WilhelmH W Spiess
Dec 22, 1999·Journal of Structural Biology·S J LudtkeW Chiu
Oct 9, 2003·Journal of the American Chemical Society·Adam LangeMarc Baldus
Jun 29, 2005·Chemical Communications : Chem Comm·Albertus P H J Schenning, E W Meijer
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Tobias JochumTeodor Silviu Balaban
May 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·Swapna GanapathyHuub J M de Groot
Jul 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Swapna GanapathyHuub J M de Groot
Aug 16, 2011·Photosynthesis Research·Anjali Pandit, Huub J M de Groot

❮ Previous
Next ❯

Citations

Jun 14, 2013·Photosynthesis Research·Gregory S Orf, Robert E Blankenship
Jul 20, 2013·Accounts of Chemical Research·Sanchita Sengupta, Frank Würthner
Jan 11, 2014·Journal of the American Chemical Society·Joonsuk HuhAlán Aspuru-Guzik
Mar 20, 2014·ACS Nano·Stéphanie ValleauAlán Aspuru-Guzik
Apr 2, 2013·Annual Review of Biophysics·A AliaJörg Matysik
Dec 7, 2013·Photochemistry and Photobiology·Yoshitaka SagaHitoshi Tamiaki
Jan 29, 2013·Biochimica Et Biophysica Acta·Gregory S OrfRobert E Blankenship
Sep 21, 2013·Biophysical Journal·Joseph Kuo-Hsiang TangAlán Aspuru-Guzik
Jan 23, 2016·Physical Chemistry Chemical Physics : PCCP·Alejandro Somoza MárquezYang Zhao
Mar 28, 2017·Scientific Reports·Tobias Kramer, Mirta Rodriguez
Apr 4, 2020·The Journal of Biological Chemistry·Donald A BryantMartin J Warren
Aug 22, 2012·Frontiers in Microbiology·Kajetan VoglDonald A Bryant
Jul 4, 2020·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Yogita SiloriArijit K De
Feb 13, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lisa M GüntherJürgen Köhler
Sep 28, 2018·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Xinmeng LiG J Agur Sevink
Apr 10, 2013·The Journal of Physical Chemistry. B·Anjali PanditHuub J M de Groot
Dec 6, 2012·The Journal of Physical Chemistry Letters·Shu FurumakiMartin Vacha

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.