Structural variation in the O-specific polysaccharides of Klebsiella pneumoniae serotype O1 and O8 lipopolysaccharide: evidence for clonal diversity in rfb genes

Molecular Microbiology
R F KellyChris Whitfield

Abstract

The O-polysaccharide fraction of the lipopolysaccharide from Klebsiella pneumoniae serotype O8 was found to comprise two galactose-containing homopolymers. Structural analysis, using chemical and high-field nuclear magnetic resonance (NMR) techniques, established that the K. pneumoniae O8 polysaccharides are composed of the linear, disaccharide repeating units [formula: see text] K. pneumoniae O8 mutant RFK-1 was isolated by resistance to phage KO1-2; strain RFK-1 expressed only D-galactan I-OAc. The 1H- and 13C-NMR resonances from this O-polysaccharide indicate that all of the O-acetyl groups within the K. pneumoniae O8 polysaccharide are carried on D-galactan I and O-acetylation occurs only on the beta-D-galactofuranose residues; 60% of the available beta-D-galactofuranose residues are non-acetylated. The O-acetylation of the remaining residues is equally distributed between the O-2 and O-6 positions. The carbohydrate backbone structures in the O8 polysaccharide are identical to D-galactan I and II expressed by K. pneumoniae O1, accounting for the antigenic cross-reaction between strains belonging to serotypes O1 and O8. However, the O1 polysaccharides are not acetylated and the O-acetyl groups present in the K. pneumoniae se...Continue Reading

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Citations

Aug 26, 2004·Antimicrobial Agents and Chemotherapy·H SahlyU Ullmann
Dec 23, 2003·Infection and Immunity·Miguel ReguéJuan M Tomás
Sep 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Leslie CuthbertsonChris Whitfield
Jun 5, 2002·Annual Review of Biochemistry·Christian R H Raetz, Chris Whitfield
Jul 21, 2005·Mediators of Inflammation·Tomislav RukavinaBrigita Ticac
Dec 3, 2014·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Hsin-An LinFeng-Yee Chang
Jan 3, 2016·International Journal of Medical Microbiology : IJMM·Valéria SzijártóGábor Nagy
Jul 16, 2013·FEMS Microbiology Reviews·Bin LiuLei Wang
Nov 28, 2008·The Journal of Biological Chemistry·Veronica KosChris Whitfield
Apr 1, 2018·The Journal of Biological Chemistry·Bradley R ClarkeChris Whitfield
Mar 30, 2017·Microbial Genomics·Rainer FolladorNicholas R Thomson
May 11, 2017·Frontiers in Microbiology·Katarina StojkovicJolanta Lukasiewicz
Apr 6, 2012·Carbohydrate Research·Laura K Greenfield, Chris Whitfield
May 1, 2021·International Journal of Molecular Sciences·Vanessa AratoFrancesca Micoli
Sep 1, 1996·Progress in Lipid Research·S G Wilkinson
Sep 25, 1997·Clinical and Diagnostic Laboratory Immunology·M TrautmannC Whitfield

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