Structural varieties of small proteoglycans in human spinal ligament

Connective Tissue Research
Masahiro YukawaMasahiko Endo

Abstract

Three types of small proteoglycan were purified from human spinal ligaments by ultracentrifugation, ion-exchange chromatography, gel-chromatography, and hydrophobic chromatography. Two of them were identified as decorin and biglycan, and the other was thought to be a decorin-subtype. Molecular sizes of decorin and decorin-subtype were both 85 kDa, and that of biglycan was 200 kDa. N-Terminal amino acid sequence of decorin-subtype corresponded with that of decorin, although it was different from decorin in terms of composition of amino acids and glycosaminoglycan chains, and reactivity with anti-human decorin antibody. The ratios of chondroitin sulfate to dermatan sulfate contained in the three proteoglycans were different, and the location of that in glycosaminoglycan chains was also thought to be different. It was demonstrated that three types of proteoglycan which are structurally different are present in extracellular matrix.

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Citations

May 19, 2004·The International Journal of Biochemistry & Cell Biology·Eiko Honda, Hiroshi Munakata
Oct 10, 2002·Biochemical and Biophysical Research Communications·Mito IwafuneKeiichi Takagaki
Dec 19, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yutaka YabeEiji Itoi
Jun 26, 2003·Neurosurgery·Natarajan Muthukumar, Usharani Karuppaswamy

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