Structurally stable Bacillus subtilis cloning vectors

Gene
L JannièreS D Ehrlich

Abstract

Cloning of long DNA segments (greater than 5 kb) in Bacillus subtilis is often unsuccessful when naturally occurring small (less than 10 kb) plasmids are used as vectors. In this work we show that vectors derived from the large (26.5 kb) plasmids pAM beta 1 and pTB19 allow efficient cloning and stable maintenance of long DNA segments (up to 33 kb). The two large plasmids differ from the small ones in several ways. First, replication of the large plasmids does not lead to accumulation of detectable amounts of ss DNA, whereas the rolling-circle replication typical for small plasmids does. In addition, the replication regions of the two large plasmids share no sequence homology with the corresponding regions of the known small plasmids, which are highly conserved. Taken together, these observations suggest that the mode of replication of the large plasmids is different from that of small plasmids. Second, short repeated sequences recombine much less frequently when carried on large than on small plasmids. This indicates that large plasmids are structurally much more stable than small ones. We suggest that the high structural stability of large plasmids is a consequence of their mode of replication and that plasmids which do not re...Continue Reading

References

Sep 1, 1979·Molecular & General Genetics : MGG·T Tanaka
Jan 1, 1979·Cold Spring Harbor Symposia on Quantitative Biology·J G Sutcliffe
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M A LovettD R Helinski
Feb 1, 1989·Journal of Bacteriology·K M DevineD J McConnell
May 1, 1989·Journal of Bacteriology·M Young, S D Ehrlich
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S Shuman
Sep 1, 1987·Molecular & General Genetics : MGG·P HaimaG Venema
Oct 1, 1987·Applied and Environmental Microbiology·D van der Lelie, G Venema
Mar 25, 1988·Cell·D BrunierS D Ehrlich
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H te RieleS D Ehrlich
Jul 5, 1987·Journal of Molecular Biology·P NoirotS D Ehrlich
Jun 1, 1988·Molecular & General Genetics : MGG·B P PeetersG Venema
Apr 20, 1988·Journal of Molecular Biology·R A SchiksnisS J Opella
Jul 1, 1985·The Journal of Antimicrobial Chemotherapy·T HoraudK Pepper
Sep 1, 1981·Plasmid·S B Primrose, S D Ehrlich
Nov 1, 1943·Genetics·S E Luria, M Delbrück

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Citations

Feb 24, 2001·Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao·J Zhou, R Petracca
Jun 15, 1992·FEMS Microbiology Letters·L OskamS Bron
Dec 1, 1991·Gene·O Arantes, D Lereclus
Dec 15, 1993·Gene·S D EhrlichB Michel
Sep 1, 1991·Research in Microbiology·S D EhrlichA Gruss
Sep 1, 1991·Research in Microbiology·S BronP Haima
Jan 9, 2004·Plasmid·Nathalie TurgeonSylvain Moineau
Aug 20, 2011·Analytical Chemistry·Armelle Cabin-FlamanVic Norris
Nov 5, 1997·Nature Biotechnology·J T Jones, B A Sullenger
Jul 15, 1998·Molecular Microbiology·V BidnenkoL Jannière
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·H YasukawaY Masamune
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M DébarbouilléG Rapoport
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C BruandL Jannière
Feb 11, 1992·Nucleic Acids Research·S Brantl, D Behnke
Sep 1, 1996·Nucleic Acids Research·L JannièreS D Ehrlich
Oct 1, 1993·The Journal of Applied Bacteriology·N CookK Killham
Oct 1, 1993·Molecular Microbiology·R KiewietJ F Seegers
Mar 1, 1992·Letters in Applied Microbiology·W GaierW P Hammes
Nov 5, 2005·Applied and Environmental Microbiology·Ana GomezMarie-Agnès Petit
Mar 31, 2004·Canadian Journal of Microbiology·Marcus SchallmeyOwen P Ward
Apr 16, 2004·BMC Microbiology·Philip A LessardAnthony J Sinskey
Oct 24, 1998·Molecular Microbiology·C Bruand, S D Ehrlich

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