Structure-Activity Relationships of Bioengineered Heparin/Heparan Sulfates Produced in Different Bioreactors

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Ha Na KimMegan S Lord

Abstract

Heparin and heparan sulfate are structurally-related carbohydrates with therapeutic applications in anticoagulation, drug delivery, and regenerative medicine. This study explored the effect of different bioreactor conditions on the production of heparin/heparan sulfate chains via the recombinant expression of serglycin in mammalian cells. Tissue culture flasks and continuously-stirred tank reactors promoted the production of serglycin decorated with heparin/heparan sulfate, as well as chondroitin sulfate, while the serglycin secreted by cells in the tissue culture flasks produced more highly-sulfated heparin/heparan sulfate chains. The serglycin produced in tissue culture flasks was effective in binding and signaling fibroblast growth factor 2, indicating the utility of this molecule in drug delivery and regenerative medicine applications in addition to its well-known anticoagulant activity.

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Citations

Dec 7, 2017·Advanced Healthcare Materials·Jelena Rnjak-KovacinaMegan S Lord
Feb 1, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Giangiacomo Torri, Giuseppe Cassinelli
Oct 26, 2018·Emerging Topics in Life Sciences·Bryan E Thacker, Susan T Sharfstein
Jan 2, 2018·Current Opinion in Biotechnology·Abinaya BadriMattheos Ag Koffas

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Methods Mentioned

BETA
ELISA
protein assay

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