PMID: 12769973Jul 1, 1997Paper

Structure-activity studies reveal two allatostatin receptor types in corpora allata of Diploptera punctata

Journal of Insect Physiology
R FeyereisenG E Pratt

Abstract

Synthetic variants of the octadecapeptide amide ASB2 (AYSYVSEYKRLPVYNFGL-NH(2)), a cockroach allatostatin, were assayed in vitro on corpora allata (CA) from 2-day-old (vitellogenic) and 10-day-old (post-vitellogenic) female Diploptera punctata. The analogs [(17)psi(18),CH(2)-S]ASB2, [D-Trp(17)]ASB2 and [Ile(18)]ASB2 inhibited juvenile hormone (JH) synthesis with simple dose-response curves on sensitive CA from 10-day-old females. These analogs were fully effective but less potent than ASB2. When tested on CA from 2-day-old mated females, which are only partially (65-70%) sensitive to ASB2, the three analogs gave biphasic dose-response curves and elicited a maximal effect only at higher concentrations. The dose-response curve for ASB2 on CA from 2-day-old females had a Hill plot slope of only 0.78+/-0.03. These findings suggested that the observed CA sensitivity to ASB2 may be the result of two partial responses having an IC(50) of approximately 0.35 and 3nM respectively. One partial response, or receptor type, appeared more sensitive than the other to adverse modification of the "message" segment of the peptide. The activity of shorter allatostatins was also studied, indicating that pentapeptides of the YXFGL-amide structure ar...Continue Reading

References

Dec 16, 1991·Biochemical and Biophysical Research Communications·M CussonS S Tobe
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S J KramerD A Schooley
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G E PrattR Feyereisen
Apr 17, 1990·Molecular and Cellular Endocrinology·G E PrattR Feyereisen
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A P WoodheadS S Tobe
Sep 29, 1989·Biochemical and Biophysical Research Communications·G E PrattR Feyereisen
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·K ReichwaldR Feyereisen
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Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H DuveA Thorpe

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Citations

Nov 6, 2001·Molecular and Cellular Endocrinology·M Meyering-VosF Sehnal
Sep 14, 2006·Annual Review of Entomology·Barbara Stay, Stephen S Tobe
Jul 6, 2000·Insect Biochemistry and Molecular Biology·L I GilbertR M Roe
May 22, 2009·Annals of the New York Academy of Sciences·R J Weaver, N Audsley

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