Structure-activity studies with endogenous allatostatins from Periplaneta americana: expressed receptor compared with functional bioassay

Journal of Insect Physiology
Gerd GädeRobert J Weaver

Abstract

The A-allatostatins (F/YXFGLamides) are insect neuropeptides with inhibitory actions on juvenile hormone (JH) synthesis, muscular contraction and vitellogenesis. They exist in multiple forms within each species. In the cockroach, Periplaneta americana, only one receptor for A-allatostatin has been identified thus far. Here, we have characterised the receptor response to all 15 of the endogenous A-allatostatins encoded by the P. americana allatostatin prohormone gene, together with some analogues, using an indirect heterologous system involving co-expression of the receptor and a potassium channel subunit in Xenopus laevis oocytes and electrophysiological measurements. We have also determined the relative potency of the same peptides to inhibit JH synthesis in corpora allata. Our data reveal that the heterologously expressed receptor responds to all of the endogenous allatostatins and, although differences in potency are recorded, this cannot readily be related to particular differences in the primary structure of the peptides. Similarly, all allatostatins act on the corpora allata to inhibit the synthesis of JH, again with varying potency not readily related to peptide structure. Interestingly, some of the peptides did not perf...Continue Reading

References

Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A P WoodheadS S Tobe
Sep 29, 1989·Biochemical and Biophysical Research Communications·G E PrattR Feyereisen
Jan 1, 1997·Peptides·H DuveA Thorpe
Jun 8, 2000·The Journal of Biological Chemistry·S R GeorgeB F O'Dowd
Jun 30, 2000·Biochemical and Biophysical Research Communications·C LenzC J Grimmelikhuijzen
Jul 13, 2000·Biochemical and Biophysical Research Communications·C LenzC J Grimmelikhuijzen
May 16, 2001·Biochemical and Biophysical Research Communications·L AuerswaldD Richter
Sep 6, 2002·Insect Biochemistry and Molecular Biology·C S GarsideS S Tobe
Sep 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Giuseppe Cazzamali, Cornelis J P Grimmelikhuijzen
Nov 26, 2002·The Journal of Biological Chemistry·Arthur ChristopoulosPatrick M Sexton
Jul 31, 2003·The European Journal of Neuroscience·Guoping FengLinda M Hall
Jan 8, 2004·EMBO Reports·Sonia Terrillon, Michel Bouvier

❮ Previous
Next ❯

Citations

Jan 13, 2009·General and Comparative Endocrinology·R Elwyn IsaacAlan D Shirras
May 22, 2009·Annals of the New York Academy of Sciences·R J Weaver, N Audsley
Sep 15, 2014·Insect Biochemistry and Molecular Biology·Juan HuangStephen S Tobe
May 20, 2015·Journal of Insect Physiology·Heleen VerlindenElisabeth Marchal
Apr 10, 2012·Journal of Proteome Research·Dries CardoenPeter Verleyen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.