Structure analysis of a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative

International Journal of Biological Macromolecules
Han YueKan Ding

Abstract

Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu), a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on. Although evidences showed polysaccharide is one of the major bioactive substances from this herb, several of them were homogenous with fine structures elucidated. In this study, we showed a novel homogeneous heteroxylan obtained from alkali-extracted crude polysaccharide. It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7. We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone. The sulfated derivative with substitution degree about 0.9 was prepared according to the chlorosulfonic acid (CSA)-pyridine method. Bioactivity tests suggested that the sulfated polysaccharide could significantly disrupt tube formation and inhibit the migration of human microvascular endothelial cells (HMEC-1) at a low concentration (0.29μM) in a...Continue Reading

Citations

Feb 14, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bo WangDong Xiang
Sep 23, 2018·International Journal of Molecular Sciences·Zhenming YuJun Duan
Feb 6, 2020·International Journal of Biological Macromolecules·Meixia LiKan Ding
Jul 2, 2021·International Journal of Biological Macromolecules·Wen-Hua ChenLu-Ping Qin
Jul 24, 2021·Carbohydrate Polymers·Mingjun ZhangJianbin Li

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