PMID: 590940Dec 1, 1977

Structure and activity of insulin, XV[1-5]. Further evidence for the importance of arginine residue B22 in the activity of insulin. Semisyntheses of despentapeptide-(B23 - 30)-insulins varied in B22 using desnonapeptide-(B22 - 30)-insulin and tetrapeptides

Hoppe-Seyler's Zeitschrift für physiologische Chemie
G WeitzelZ Rehe

Abstract

Insulin hexamethyl ester was digested by trypsin. The resulting desoctapeptide-(B23 - 30)-insulin pentamethyl ester was purified. This compound was digested by carboxypeptidase B to remove the arginine residue B22 at the end of the B chain. Then the N-terminal amino groups of the remaining desnonapeptide-(B22 - 30)-insulin pentamethyl ester were protected with the Boc residue. The free carboxyl group of the glutamic acid residue B21 of this product was coupled to the following synthetic tetrapeptide esters: Arg-Gly-Phe-Phe-OMe, Lys(Boc)-Gly-Phe-Phe-OMe, Orn(Boc)-Gly-Phe-Phe-OMe, Cit-Gly-Phe-Phe-OMe, Ala-Gly-Phe-Phe-OMe and Gly-Gly-Phe-Phe-OMe. The syntheses of these peptide esters are described. After removal of all protecting groups, despentapeptide-insulin (B22-Arg) and analogues of this product with variation in position B22 could be obtained. They were purified by column chromatography. The biological activities of these components were determined by the mouse fall test. In the case of despentapeptide insulin (C-terminus Arg-Gly-Phe-Phe), the activity rose to the expected value of 34%. The insulin variants with amino acid residues other than arginine in position B22 had much lower activities: with lysine 13%, with ornithine...Continue Reading

References

Feb 1, 1976·Hoppe-Seyler's Zeitschrift für physiologische Chemie·G WeitzelK Eisele
Mar 1, 1973·Hoppe-Seyler's Zeitschrift für physiologische Chemie·G WeitzelW Stock
Jun 1, 1955·Biochimica Et Biophysica Acta·L A SLUYTERMAN

Citations

Jul 25, 1979·Journal of Molecular Biology·J F CutfieldC D Reynolds
Apr 6, 2007·Biopolymers·John P MayerRichard D DiMarchi
Jul 1, 1978·Die Naturwissenschaften·F Schneider
Dec 1, 1982·Hoppe-Seyler's Zeitschrift für physiologische Chemie·R KnorrC R Kahn

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