Structure and antimicrobial activity relationship of royalisin, an antimicrobial peptide from royal jelly of Apis mellifera

Peptides
Katarina BílikovaChi-Chung Peng

Abstract

Royalisin is a 5.5-kDa antibacterial peptide isolated from the royal jelly of the honeybee (Apis mellifera). The antimicrobial activity of royalisin against fungi, Gram-positive and Gram-negative bacteria has been revealed. Compared with another insect antibacterial peptide, there is an extra stretch of 11 amino acid residues at the C-terminus of royalisin. In this study, a recombinant shortened form of royalisin named as royalisin-D, was constructed without the 11 amino acid residues at the C-terminal of royalisin and linked to the C-terminal of oleosin by an inteinS fragment. The recombinant protein was overexpressed in Escherichia coli, purified by artificial oil body system and subsequently released through self-splicing of inteinS induced by the changes of temperature. The antibacterial activity of royalisin-D was compared with royalisin via minimal inhibitory concentration (MIC) assay, minimal bactericidal concentration (MBC) assay, microbial adhesion to solvents (MATS) methods, and cell membrane permeability. Furthermore, the recombinant royalisin and royalisin-D have also been treated with the reducing agent of disulfide bonds, dithiothreitol (DTT), to investigate the importance of the intra-disulfide bond in royalisin....Continue Reading

References

Jul 30, 1999·Developmental and Comparative Immunology·P BuletD Hoffmann
Feb 19, 2002·International Journal of Antimicrobial Agents·A SpecialeG Nicoletti
Mar 5, 2003·Pharmacological Reviews·Michael R Yeaman, Nannette Y Yount
Dec 4, 2003·Biochimica Et Biophysica Acta·Yoshiro NakajimaMinoru Yamakawa
Jan 7, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·K Poole
Dec 21, 2004·Insect Biochemistry and Molecular Biology·Jaroslav KlaudinyJozef Simúth
Jan 11, 2005·Protein and Peptide Letters·Philippe Bulet, Reto Stöcklin
Feb 11, 2005·Nature Reviews. Microbiology·Kim A Brogden
May 12, 2006·The Journal of Antimicrobial Chemotherapy·H AltmanG Bachrach
Jul 10, 2007·Journal of Industrial Microbiology & Biotechnology·Jon PalmerJohn Brooks
Aug 19, 2009·The Journal of Antimicrobial Chemotherapy·David M Livermore
Nov 19, 2011·IUBMB Life·Paulus H S Kwakman, Sebastian A J Zaat

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Citations

Feb 11, 2016·Journal of Medical Microbiology·Martin SojkaJuraj Majtan
Sep 25, 2016·Microbiological Research·Filippo FratiniAntonio Felicioli
Sep 2, 2016·Journal of Biomolecular Structure & Dynamics·Marco FranzoiStefano Mammi
Feb 1, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Katarzyna GreckaPiotr Szweda
Mar 31, 2018·Journal of Peptide Science : an Official Publication of the European Peptide Society·Akira SatoKeiichi Ebina
Aug 11, 2017·BMC Complementary and Alternative Medicine·Chi-Chung PengJen-Chieh Li
Jul 5, 2018·BMC Complementary and Alternative Medicine·Yuan-Chang YangChi-Chung Peng
Jan 16, 2020·International Journal of Molecular Sciences·Saboor AhmadJianke Li
Sep 6, 2018·Journal of Economic Entomology·Praetinee PattamayutanonPanuwan Chantawannakul
Jan 19, 2019·Scientific Reports·Xueqing ZhangChengqiang Feng
Jan 10, 2020·Journal of Animal Physiology and Animal Nutrition·Sameh A AbdelnourAyman A Swelum
Dec 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mária ŠediváJaroslav Klaudiny
Feb 11, 2021·Nutrients·Nicolas CollazoMiguel A Prieto
Jan 20, 2021·Proteomics·Vladimir N UverskyElrashdy M Redwan
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eliza MatuszewskaJan Matysiak
May 18, 2021·Pesticide Biochemistry and Physiology·Tingting ZhangJianzhen Zhang
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Magdalena RatajczakJan Matysiak
Nov 23, 2021·Journal of Agricultural and Food Chemistry·Shanshan LiFuliang Hu

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