Structure and biological activity of the transcriptional initiation sequences of the murine c-myb oncogene.

Nucleic Acids Research
P W SobieszczukA R Dunn

Abstract

To study the control mechanism(s) that govern the transcription of c-myb, genomic clones corresponding to the 5' region of the murine c-myb gene have been isolated and characterized structurally and functionally. Primer extension and nuclease protection analysis have revealed the presence of multiple transcriptional initiation sites, that are utilized in several hemopoietic cell lines (WEHI3B(D+). FDC-P1 and RB22.2). Some of the sites are used in all cell lines but others are unique; all are located in a region of the c-myb gene that is G-C rich, contains a number of potential Sp1 binding sites and lacks classical promoter consensus sequences. Experiments in which well characterized promoters controlling expression of a reporter gene have been replaced by fragments of c-myb DNA (including the observed cap sites) were performed in an attempt to demonstrate promoter activity in various cell types. It was shown that a region of the c-myb gene (approximately 1.0 kbp upstream from the splice donor site of the first exon) contains a weak promoter that has a low level of transcriptional activity in hemopoietic as well as in fibroblastic cells. These results support the suggestion that c-myb expression is not regulated primarily at the...Continue Reading

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Citations

Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·C Abbott
Jun 1, 1993·Molecular Immunology·J D Mountz, M A Subler
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Z RatajczakA M Gewirtz
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G Sáfrány, R P Perry
Jan 5, 2011·Frontiers in Bioscience (Landmark Edition)·Ye Zhou, Scott A Ness
Mar 31, 1995·The Journal of Biological Chemistry·C R TothT P Bender

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