Structure and capsid association of the herpesvirus large tegument protein UL36.

Journal of Virology
William W Newcomb, Jay C Brown

Abstract

The tegument of all herpesviruses contains a high-molecular-weight protein homologous to herpes simplex virus (HSV) UL36. This large (3,164 amino acids), essential, and multifunctional polypeptide is located on the capsid surface and present at 100 to 150 copies per virion. We have been testing the idea that UL36 is important for the structural organization of the tegument. UL36 is proposed to bind directly to the capsid with other tegument proteins bound indirectly by way of UL36. Here we report the results of studies carried out with HSV type 1-derived structures containing the capsid but lacking a membrane and depleted of all tegument proteins except UL36 and a second high-molecular-weight protein, UL37. Electron microscopic analysis demonstrated that, compared to capsids lacking a tegument, these capsids (called T36 capsids) had tufts of protein located at the vertices. Projecting from the tufts were thin, variably curved strands with lengths (15 to 70 nm) in some cases sufficient to extend across the entire thickness of the tegument (approximately 50 nm). Strands were sensitive to removal from the capsid by brief sonication, which also removed UL36 and UL37. The findings are interpreted to indicate that UL36 and UL37 are t...Continue Reading

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Citations

Aug 24, 2012·Journal of Virology·William W Newcomb, Jay C Brown
Feb 15, 2013·Journal of Virology·Daniel HenaffRoger Lippé
Oct 7, 2011·Veterinary Research·Steven J van BeurdenAlain F Vanderplasschen
Jun 26, 2012·Annual Review of Microbiology·Gregory Smith
Jan 14, 2016·Virology·Nora F MahmoudYasuko Mori
Sep 24, 2015·Viruses·Danielle J OwenStephen C Graham
May 29, 2012·Trends in Microbiology·Ritesh Tandon, Edward S Mocarski
Dec 15, 2012·The Journal of General Virology·Gen TakahashiYoshiaki Yura
Jun 15, 2012·Virology·William W NewcombJay C Brown
Jan 23, 2015·Nature Communications·Romain F LaineClemens F Kaminski
Jun 15, 2018·Journal of Virology·Gina R DanielGregory A Smith
Apr 16, 2011·Nature Reviews. Microbiology·David C Johnson, Joel D Baines
Apr 26, 2016·Nature Structural & Molecular Biology·Alexis HuetJames F Conway
Oct 3, 2019·MSystems·Anna Hernández DuránMaya Topf
Nov 6, 2018·Frontiers in Microbiology·Francisco J IbáñezPablo A González
Aug 23, 2020·International Journal of Molecular Sciences·Imran Ahmad, Duncan W Wilson
Nov 27, 2020·Journal of Virology·Nabil El BilaliRoger Lippé

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