PMID: 6978813Apr 1, 1982Paper

Structure and catalytic inactivity of the bacterial luciferase neutral flavin radical

European Journal of Biochemistry
M KurfürstJ W Hastings

Abstract

A luciferase-bound neutral flavin semiquinone radical can be formed upon the oxidation of the luciferase-FMNH2 complex by molecular oxygen. This species can also be formed anaerobically by comproportionation of FMN and FMNH2 in the presence of luciferase. The radical is kinetically stable (t1/2 approximately 20 h at 0 degree C in air; the Arrhenius delta H not equal to decay being about 170 kJ/mol) and can be prepared in pure form by Sephadex G-25 chromatography at 0-4 degrees C. The pure enzyme-bound radical is inactive for light emission either with or without aldehyde, and is not in (relevantly rapid) equilibrium with the luciferase 4a-peroxyflavin, the active intermediate in the bioluminescent reaction.

References

Jun 1, 1977·European Journal of Biochemistry·S GhislaM Husein
Aug 1, 1977·Journal of Bacteriology·C A Waters, J W Hastings
Jan 1, 1977·Annual Review of Microbiology·J W Hastings, K H Nealson
May 2, 1978·Biochemistry·J E BecvarJ W Hastings
Jul 1, 1979·Photochemistry and Photobiology·R P Presswood, J W Hastings
Jun 14, 1978·Biochemical and Biophysical Research Communications·R Presswood, J W Hastings
Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·C Kemal, T C Bruice
Oct 1, 1967·European Journal of Biochemistry·A EhrenbergP Hemmerich
Feb 1, 1972·European Journal of Biochemistry·F MüllerW H Walker
Jan 1, 1971·Analytical Biochemistry·G W Mitchell, J W Hastings
Jan 1, 1969·Photochemistry and Photobiology·J Koziol
Jul 1, 1980·Archives of Biochemistry and Biophysics·M V Merritt, T O Baldwin
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Apr 20, 1981·FEBS Letters·J Geissler, P Hemmerich
Jan 29, 1980·Biochemical and Biophysical Research Communications·E M Kosower
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Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J W HastingsP Douzou
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S GhislaJ M Lhoste

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Citations

Jul 1, 1990·Journal of Bioluminescence and Chemiluminescence·A D IsmailovV S Danilov
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M KurfürstJ W Hastings
Aug 1, 1983·Photochemistry and Photobiology·I B Matheso, J Lee
Jun 2, 2011·Birth Defects Research. Part A, Clinical and Molecular Teratology·George R FlentkeSusan M Smith
Oct 1, 1995·Proteins·T Sandalova, Y Lindqvist

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