Structure and cellular dynamics of Deinococcus radiodurans single-stranded DNA (ssDNA)-binding protein (SSB)-DNA complexes.

The Journal of Biological Chemistry
Nicholas P GeorgeJames L Keck

Abstract

The single-stranded DNA (ssDNA)-binding protein from the radiation-resistant bacterium Deinococcus radiodurans (DrSSB) functions as a homodimer in which each monomer contains two oligonucleotide-binding (OB) domains. This arrangement is exceedingly rare among bacterial SSBs, which typically form homotetramers of single-OB domain subunits. To better understand how this unusual structure influences the DNA binding and biological functions of DrSSB in D. radiodurans radiation resistance, we have examined the structure of DrSSB in complex with ssDNA and the DNA damage-dependent cellular dynamics of DrSSB. The x-ray crystal structure of the DrSSB-ssDNA complex shows that ssDNA binds to surfaces of DrSSB that are analogous to those mapped in homotetrameric SSBs, although there are distinct contacts in DrSSB that mediate species-specific ssDNA binding. Observations by electron microscopy reveal two salt-dependent ssDNA-binding modes for DrSSB that strongly resemble those of the homotetrameric Escherichia coli SSB, further supporting a shared overall DNA binding mechanism between the two classes of bacterial SSBs. In vivo, DrSSB levels are heavily induced following exposure to ionizing radiation. This accumulation is accompanied by dra...Continue Reading

References

Jan 1, 1984·Cold Spring Harbor Symposia on Quantitative Biology·J D GriffithJ Register
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S Chrysogelos, J Griffith
Jun 30, 1982·Biochimica Et Biophysica Acta·S Kitayama
Jan 1, 1997·Annual Review of Microbiology·J R Battista
Aug 10, 2000·Nature Structural Biology·S RaghunathanG Waksman
Jan 11, 2003·Science·Smadar Levin-ZaidmanAbraham Minsky
Oct 8, 2003·The Journal of Biological Chemistry·Nami HarutaMichael M Cox
Jan 14, 2004·BMC Microbiology·Julie Malia EggingtonMichael Matthew Cox
May 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Douglas A BernsteinJames L Keck
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Nov 2, 2005·Nature Reviews. Microbiology·Michael M Cox, John R Battista
Sep 29, 2006·Nature·Ksenija ZahradkaMiroslav Radman
Oct 24, 2006·Acta Crystallographica. Section D, Biological Crystallography·Robert JedrzejczakJózef Kur
Dec 11, 2007·Biophysical Journal·Gregor WitteUte Curth
Dec 15, 2007·Nature Protocols·Robert A GrassucciJoachim Frank
Jun 24, 2008·Critical Reviews in Biochemistry and Molecular Biology·Melanie BlasiusUlrich Hübscher
Oct 22, 2008·Critical Reviews in Biochemistry and Molecular Biology·Robert D SheredaJames L Keck
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Jun 12, 2009·The Journal of Biological Chemistry·Cédric A NoraisMichael M Cox
Feb 5, 2010·Nucleic Acids Research·Seiji Sugiman-Marangos, Murray S Junop
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Dea Slade, Miroslav Radman
Oct 13, 2011·Molecular & Cellular Proteomics : MCP·Bhakti Basu, Shree Kumar Apte
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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Citations

Aug 28, 2014·BioMed Research International·Yen-Hua Huang, Cheng-Yang Huang
Nov 10, 2015·Biochimica Et Biophysica Acta·Venkatasubramanian VidhyasagarYuliang Wu
Sep 12, 2013·Journal of Molecular Biology·Edwin AntonyTimothy M Lohman
Jul 26, 2015·BMC Microbiology·Guozhi DaiYimou Wu
Apr 17, 2016·Nucleic Acids Research·Vincent M WaldmanTimothy M Lohman
Feb 24, 2015·Journal of Biochemistry·Takahiko AramakiTadashi Ueda
Jul 5, 2013·Structure·Thayne H DickeyDeborah S Wuttke
Aug 4, 2016·Nucleic Acids Research·Tricia A Windgassen, James L Keck
Jun 14, 2017·Nucleic Acids Research·Christine ToumaRoland Gamsjaeger
Dec 30, 2018·The Journal of Biological Chemistry·Tricia A WindgassenJames L Keck
Apr 5, 2019·Protein Science : a Publication of the Protein Society·Aaron J Oakley
Aug 30, 2014·The Journal of Biological Chemistry·Mikhail RyzhikovSergey Korolev
Apr 24, 2015·The Journal of Biological Chemistry·Christine PetzoldJames L Keck
Nov 17, 2015·Biochemistry. Biokhimii︠a︡·A A Agapov, A V Kulbachinskiy
Jul 28, 2018·Nucleic Acids Research·Debjani BagchiVincent Croquette
Jul 7, 2016·The Journal of Biological Chemistry·Sarah R WesselJames L Keck
Sep 12, 2018·Proceedings of the National Academy of Sciences of the United States of America·Tricia A WindgassenJames L Keck
Feb 27, 2021·Biophysical Journal·Alexander G Kozlov, Timothy M Lohman
Apr 2, 2015·The Journal of Physical Chemistry. B·Kaushik Chakraborty, Sanjoy Bandyopadhyay

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