Structure and characterization of rat cyclin D3 promoter

Gene
M YangK Nakashima

Abstract

To investigate the transcription initiation mechanism of the gene encoding cyclin D3, one of the major G1 cyclins to promote the G1/S transition, we cloned the 5' portion of rat cyclin D3 gene and analyzed the promoter region. The major transcription start point of the gene was identified by the primer extension experiment to be 207 bp upstream from the ATG start codon and its promoter region was found to have no canonical TATA box. The DNA mobility shift assay and DNase I footprinting analysis using nuclear extracts from Nb2 cells stimulated by prolactin (PRL) have clearly revealed that the promoter possesses two binding sites for the PRL-induced transcription factors. One is located between -1 and -59 bp (Region I) and the other between -328 and -380 bp (Region II). The induction of transcription factors by PRL was blocked by simultaneous addition of cycloheximide, suggesting that protein synthesis was necessary for the release of these PRL-responsive transcription factors. Region I contained putative binding elements for ATF/CREB, SP1 and AP2, and Region II contained elements for a MCBF/MGF and a novel factor. These results suggest that the cyclin D3 gene is a TATA-less gene whose transcription is regulated by multiple mitog...Continue Reading

References

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Citations

Nov 20, 2002·Biochemical and Biophysical Research Communications·Masayo FujitaSatoshi Inoue
Feb 12, 1998·Biochemical and Biophysical Research Communications·M Kobayashi, K Kawakami
May 4, 2011·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·E A NeriN A Rebouças
Mar 4, 2003·The Journal of Biological Chemistry·Yihong MaW Douglas Cress
Oct 17, 1998·Molecular and Cellular Biology·M RahmatullahD J Carey
Feb 23, 2021·Cancer Treatment and Research Communications·Jan PawlonkaUrszula Ambroziak
May 12, 2009·The Journal of Steroid Biochemistry and Molecular Biology·Noemi Baranda-AvilaMarco Cerbón

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