Structure and complete nucleotide sequence of the gene encoding rat fibroblast tropomyosin 4

Journal of Molecular Biology
J P Lees-MillerD M Helfman

Abstract

We have isolated and determined the complete nucleotide sequence of the gene that encodes the 248 amino acid residue fibroblast tropomyosin, TM-4. The TM-4 sequence is encoded by eight exons, which span approximately 16,000 bases. The position of the intron-exon splice junctions relative to the final transcript are identical to those present in other vertebrate tropomyosin genes and the Drosophila melanogaster TMII gene. We have found no evidence that the rat TM-4 gene is alternatively spliced, unlike all the other tropomyosin genes from multicellular organisms that have been described. Typical vertebrate tropomyosin genes contain some, or all, of alternatively spliced exons 1a and 1b, 2a and 2b, 6a and 6b, and 9a, 9b, 9c and 9d in addition to common exons 3, 4, 5, 7 and 8. The rat fibroblast TM-4 mRNA is encoded by sequences most similar to exons 1b, 3, 4, 5, 6b, 7, 8 and 9d. Two exon-like sequences that are highly similar to alternatively spliced exons 2b and 9a of the rat beta-tropomyosin gene and the human TMnm gene have been located in the appropriate region of the gene encoding rat fibroblast TM-4. However, several mutations in these sequences render them non-functional as tropomyosin coding exons. We have termed these ex...Continue Reading

References

Aug 1, 1988·The Journal of Cell Biology·J J LinJ L Lin
Jun 1, 1986·Molecular and Cellular Biology·C C Karlik, E A Fyrberg
Dec 1, 1988·Molecular and Cellular Biology·K TakenagaS Sakiyama
Jan 1, 1988·Molecular and Cellular Biology·A R MacLeod, C Gooding
Jul 1, 1986·Molecular and Cellular Biology·J LeavittS Burbeck
Dec 1, 1984·Molecular and Cellular Biology·H HamadaC M Gorman
Jan 1, 1980·Annual Review of Biochemistry·R S Adelstein, E Eisenberg
Aug 1, 1982·European Journal of Biochemistry·A R MacLeod

❮ Previous
Next ❯

Citations

Sep 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J P Lees-Miller, D M Helfman
Jul 24, 2002·Virus Research·Jeong-Ah Kang, Ann W Funkhouser
Mar 13, 2010·European Journal of Cell Biology·Thomas FathPeter Gunning
Feb 1, 1994·Current Opinion in Cell Biology·M F PittengerD M Helfman
Nov 10, 2011·Bioarchitecture·Galina SchevzovPeter W Gunning
Feb 10, 2004·The Journal of Physiology·Robert D GaffinMariappan Muthuchamy
Jan 1, 1995·Nature Genetics·H J JacobA Lernmark
Jan 16, 2008·Physiological Reviews·Peter GunningEdna Hardeman
Dec 22, 1995·The Journal of Biological Chemistry·M MuthuchamyD F Wieczorek
Aug 9, 2002·The Journal of Biological Chemistry·Adriana A PaulucciChuck S Farah
Feb 1, 1992·Journal of Muscle Research and Cell Motility·D E FleenorR B Devlin
Jun 1, 1993·Molecular and Cellular Biology·M MuthuchamyD F Wieczorek
Oct 30, 2003·European Journal of Cell Biology·Marouan AbouhamedGabriele Plenz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.