Structure and diversity of the human T-cell receptor beta-chain variable region genes

Science
J P TillinghastD Y Loh

Abstract

In order to characterize the variability of the expressed human T-cell receptor (TCR) beta-chain repertoire and contrast this variability to the known murine beta-chain repertoire, 15 independent complementary DNA (cDNA) clones containing TCR beta-chain variable region (V beta) genes were isolated from a human tonsil cDNA library. The nucleotide and derived amino acid sequences of these 15 V beta genes were analyzed together with 7 previously defined sequences. Fifteen different human V beta genes could be identified from 22 independent sequences. By means of DNA hybridization and sequence homology comparisons, it was possible to group these 15 genes into ten distinct V beta subfamilies, each containing from one to seven members. Minimal polymorphism was noted between individuals, except in multimember subfamilies. The amino acid sequences of these genes contain conserved amino acids that are also shared by murine TCR V beta genes and immunoglobulins; no features were found that distinguish human V beta genes from their murine counterparts. Evaluation of secondary structure showed that maximum variability coincides with generally hydrophilic portions of the amino acid sequence, while specific hydrophobic regions were conserved ...Continue Reading

References

Jan 1, 1979·Annual Review of Biochemistry·L M Amzel, R J Poljak
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1977·Cold Spring Harbor Symposia on Quantitative Biology·M Weigert, R Riblet
May 1, 1985·The Journal of Experimental Medicine·R EpsteinM Cohn
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M A BehlkeD Y Loh
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J NovotnýH N Eisen
Sep 1, 1986·The Journal of Experimental Medicine·N KimuraT W Mak
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A TunnacliffeT H Rabbitts
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K IkutaT Honjo
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B ToyonagaT W Mak
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J Novotný, E Haber
Aug 1, 1974·European Journal of Immunology·G M Shearer
Apr 14, 1983·Nature·S Tonegawa
Jan 1, 1983·Annual Review of Immunology·D R Davies, H Metzger
Nov 1, 1984·Nature·Y ChienM M Davis

❮ Previous
Next ❯

Citations

Nov 15, 1996·International Journal of Cancer. Journal International Du Cancer·A JureticG C Spagnoli
Apr 1, 1991·Clinica Chimica Acta; International Journal of Clinical Chemistry·J J van Dongen, I L Wolvers-Tettero
Jan 21, 1992·Journal of Immunological Methods·A BettinardiD Primi
Jun 1, 1991·Molecular Immunology·P J DohertyA Cohen
Aug 1, 1993·Journal of Neuroimmunology·E Martínez-NavesC López-Larrea
Aug 1, 1995·Immunology and Cell Biology·C Olive
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M H KleinL Hood
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P CharmleyR A Gatti
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R JoresT Meo
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R BaccalaA N Theofilopoulos
Apr 1, 1987·The Journal of Experimental Medicine·P ConcannonL E Hood
Feb 1, 1988·The Journal of Experimental Medicine·M L ClearyS D Smith
Oct 1, 1989·The Journal of Experimental Medicine·E SebounS L Hauser
Jan 1, 1990·The Journal of Experimental Medicine·Y LiD N Posnett
Oct 1, 1991·The Journal of Experimental Medicine·B TyckoJ Sklar
Dec 1, 1991·The Journal of Experimental Medicine·Y LiD N Posnett
Aug 1, 1993·Scandinavian Journal of Immunology·J CihakH W Ziegler-Heitbrock
Jul 7, 1995·Annals of the New York Academy of Sciences·P Concannon, M A Robinson
Jan 1, 1987·Nucleic Acids Research·J Schmidtke, D N Cooper
Dec 30, 1991·Annals of the New York Academy of Sciences·D A HaflerH L Weiner

❮ Previous
Next ❯

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