Structure and dynamics of bacteriophage IKe major coat protein in MPG micelles by solution NMR

Biochemistry
K A WilliamsL E Kay

Abstract

The structure and dynamics of the 53-residue filamentous bacteriophage IKe major coat protein in fully protonated myristoyllysophosphatidylglycerol (MPG) micelles were characterized using multinuclear solution NMR spectroscopy. Detergent-solubilized coat protein [sequence: see text] mimics the membrane-bound "assembly intermediate" form of the coat protein which occurs during part of the phage life cycle. NMR studies of the IKe coat protein show that the coat protein is largely alpha-helical, exhibiting a long amphipathic surface. helix (Asn 4 to Ser 26) and a shorter "micelle-spanning" C-terminal helix which begins at TRP 29 and continues at least to Phe 48. Pro 30 likely occurs in the first turn of the C-terminal helix, where it is ideally situated given the hydrogen bonding and steric restrictions imposed by this residue. The similarity of 15N relaxation values (T1, T2, and NOE and 500 MHz and T2 at 600 MHz) among much of the N-terminal helix and all of the TM helix indicates that the N-terminal helix is as closely associated with the micelle as the TM helix. The description of the protein in the micelle is supported by the observation of NOEs between lysolipid protons and protein amide protons between asn 8 and Ser 50. The ...Continue Reading

References

Jan 1, 1979·Journal of Supramolecular Structure·T A Cross, S J Opella
Mar 20, 1991·Journal of Molecular Biology·M W MacArthur, J M Thornton
Jul 5, 1990·Journal of Molecular Biology·D E StewartJ E Wampler
Jul 1, 1970·Journal of the American Chemical Society·A Veis, C F Nawrot
May 1, 1994·Journal of Biomolecular NMR·A M Gronenborn, G M Clore
Feb 1, 1994·European Journal of Biochemistry· Orekhov VYuA S Arseniev
Oct 15, 1993·Biochemical and Biophysical Research Communications·K A Williams, C M Deber
Oct 5, 1993·Journal of Molecular Biology·P A McDonnellS J Opella
Feb 5, 1993·Journal of Molecular Biology·C Landolt-MarticorenaR A Reithmeier
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax

❮ Previous
Next ❯

Citations

Dec 24, 1997·Matrix Biology : Journal of the International Society for Matrix Biology·E E Marcantonio, F S David
Nov 5, 1997·Current Opinion in Structural Biology·A G Palmer
Jun 19, 1998·Current Opinion in Structural Biology·D A Marvin
Nov 18, 1998·Current Opinion in Structural Biology·F M Marassi, S J Opella
May 11, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Tyler Reddy, Jan K Rainey
Apr 9, 2008·Annual Review of Physical Chemistry·Stanley J OpellaSang Ho Park
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·J Klein-SeetharamanH G Khorana
May 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ana Carolina ZeriStanley J Opella
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·César FernándezKurt Wüthrich
Feb 18, 2010·Progress in Nuclear Magnetic Resonance Spectroscopy·Hak Jun KimCharles R Sanders
Jul 11, 2006·Magnetic Resonance in Chemistry : MRC·Charles R Sanders, Frank Sönnichsen
Jul 14, 2001·Chemistry & Biology·L OlofssonI A Nicholls
Nov 24, 2007·Biochimica Et Biophysica Acta·Marc WittlichDieter Willbold
Mar 31, 2004·The Journal of Biological Chemistry·R Scott HoulistonJames H Davis
Mar 2, 2019·Proceedings of the National Academy of Sciences of the United States of America·Jingwei XuYe Xiang
May 1, 1997·The Journal of Peptide Research : Official Journal of the American Peptide Society·M R Tessmer, D A Kallick
Nov 30, 2019·Progress in Nuclear Magnetic Resonance Spectroscopy·Amir Goldbourt
May 11, 2006·Chemical Reviews·Kevin R Mackenzie

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.