Structure and expression of a cloned cDNA for human (2'-5')oligoadenylate synthetase

Journal of Biochemistry
S ShiojiriY Sokawa

Abstract

17S poly(A)+RNA, which hybridized to an oligonucleotide complementary to a part of the partial cDNA (E1cDNA) (Merlin et al. (1983) Proc. Natl. Acad. Sci. U.S. 80, 4904) for 2-5A synthetase, was isolated from interferon-treated human KB cells and used for cDNA cloning. Several overlapping cDNAs were cloned by using the oligonucleotide as a probe. Two of them were joined at their overlapping region, resulting in a cDNA (22-1 cDNA) of 1.4 kb containing a long open reading frame. When the cDNA was expressed in COS-7 cells with an eukaryotic promoter, active 2-5A synthetase was produced and localized mainly in the cytoplasm. The 5'-proximal ATG in 22-1 cDNA is followed immediately by another ATG. This second ATG was assumed to work as the initiator codon. If so, this enzyme comprises 363 amino acids.

Citations

Jul 1, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Y HariyaN Fujii
Jun 1, 1994·Journal of Interferon Research·Y SokawaK Takeda
Oct 1, 1993·Journal of Interferon Research·O López OcejoP López Saura
May 20, 1998·Biochemical and Biophysical Research Communications·A MoroS E Perea
Jun 12, 1998·Pharmacology & Therapeutics·M R Player, P F Torrence

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