Structure and expression of mitochondrial citrate synthases from higher plants

Plant & Cell Physiology
U La CognataB Müller-Röber

Abstract

Mitochondrial citrate synthase (EC 4.1.3.7) represents the first enzyme of the tricarboxylic acid cycle, catalyzing the condensation of acetyl-CoA and oxaloacetate, finally yielding citrate and CoA. We report here the isolation of cDNA clones encoding citrate synthase from Nicotiana tabacum, Beta vulgaris and Populus. Nucleotide and deduced amino acid sequences were compared with previously published sequences of mitochondrial citrate synthases from Arabidopsis thaliana and potato, as well as with the sequence of glyoxysomal citrate synthase from pumpkin. Homologies between the various plant mitochondrial enzymes were in the range from 77.2% (potato vs. Arabidopsis) to 94.2% (potato vs. tobacco) on the nucleotide level (coding regions only), and in the range from 70.1% to 90.4% (potato vs. Arabidopsis, and potato vs. tobacco, respectively) on the amino acid level. Identities of the mitochondrial isozymes to the pumpkin glyoxysomal enzyme were below 30% on the nucleotide and amino acid level. In Northern blot experiments citrate synthase mRNA was detected in all tissues analyzed. However, levels of expression showed tissue dependency despite the fact that citrate synthase is usually considered a house-keeping enzyme. Whether the...Continue Reading

Citations

Nov 29, 2002·The EMBO Journal·Gwenaël Ruprich-RobertMarguerite Picard
Feb 22, 2011·Annual Review of Plant Biology·A Harvey MillarDavid A Day
Sep 11, 2013·Bioscience, Biotechnology, and Biochemistry·Shinji WakutaHirokazu Matsui
Dec 27, 2015·Plant Science : an International Journal of Experimental Plant Biology·Zhenbin LiuDongtao Ren
Feb 4, 2003·Anais Da Academia Brasileira De Ciências·Marcelo J SurpiliLothar Willmitzer
Jan 1, 2008·The Arabidopsis Book·A Harvey MillarJames Whelan
Apr 12, 2020·Mitochondrion·Abir U Igamberdiev

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