Structure and expression of the ATFa gene.

The Journal of Biological Chemistry
J GoetzClaude Kedinger

Abstract

The human ATFa proteins belong to the ATF/CREB family of transcription factors. We have previously shown that they mediate the transcriptional activation by the largest E1a protein and can heterodimerize with members of the Jun/Fos family. ATFa proteins have also been found tightly associated with JNK2, a stress-activated kinase. We now report on the structure of the ATFa gene, which mapped to chromosome 12 (band 12q13). Sequence analysis revealed that ATFa isoforms are generated by alternative splice donor site usage. A minimal promoter region of approximately 200 base pairs was identified that retained nearly full transcriptional activity. Binding sites for potential transcription factors were delineated within a GC-rich segment by DNase I footprinting. Expression studies revealed that ATFa accumulates in the nuclei of transfected cells, and the nuclear localization signal was defined next to the leucine zipper domain. As revealed by hybridization with mouse ATFa sequences, low levels of ATFa mRNAs were ubiquitously distributed in fetal or adult mice, with enhanced expression in particular tissues, like squamous epithelia and specific brain cell layers. The possible significance of coexpression of ATFa, ATF-2, and Jun at simi...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Apr 1, 1992·Current Opinion in Genetics & Development·P K Brindle, M R Montminy
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K Chida, P K Vogt
Dec 1, 1991·Trends in Biochemical Sciences·C Dingwall, R A Laskey
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T Hai, T Curran
Apr 7, 1989·Cell·S T Smale, D Baltimore
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard
Jul 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·B M HagmeyerH van Dam
Nov 1, 1994·Developmental Biology·C MendelsohnP Chambon
May 1, 1994·Journal of Cellular Biochemistry·T Boulikas
Jan 1, 1994·Cytogenetics and Cell Genetics·D J DemetrickD H Beach
Jan 19, 1996·The Journal of Biological Chemistry·G LiangT Hai
Jan 1, 1995·Annual Review of Cell and Developmental Biology·P Sassone-Corsi

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Citations

Dec 20, 2002·DNA and Cell Biology·Peter StoilovStefan Stamm
Mar 3, 2012·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Ki-Hyeon SeongShunsuke Ishii
Mar 7, 2006·Peptides·Johanna M NystedtPertti Panula
Jan 21, 2012·Biochemical Society Transactions·Malgorzata Gozdecka, Wolfgang Breitwieser
Jul 23, 2014·Mediators of Inflammation·Tao YuJae Youl Cho
Oct 28, 2008·Journal of Molecular Biology·Barbara CamuzeauxBruno Chatton
Jun 22, 1999·Oncogene·F De GraeveB Chatton
Mar 1, 2005·Oncogene·Pierre-Jacques HamardBruno Chatton
May 21, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Chengtian ZhaoAnming Meng
Jun 6, 1998·The Journal of Biological Chemistry·R V GopalkrishnanC Kedinger
Aug 30, 2019·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Xiao-Shuang WangDe-Shou Wang

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