Structure and expression of the gene encoding a Broad-Complex homolog in the silkworm, Bombyx mori

Gene
Yoshinori Nishita, Shigeharu Takiya

Abstract

The steroid hormone ecdysone (20-hydroxyexdysone) initiates metamorphosis and also larval ecdysis in many insects by activating a cascade of genes that includes primary response genes (early genes), most of which encode transcriptional regulators, and secondary response genes (late genes) regulated by the early genes. One of the early genes, Broad-Complex (BR-C), a key regulator of the ecdysone cascade, shares a common amino-terminal BTB domain which is fused by alternative splicing to one of four pairs of C(2)H(2) zinc finger domains (Z1, Z2, Z3, and Z4). cDNAs for BR-C (BmBR-C) were isolated from the silkworm Bombyx mori. These genes showed 90.3% and 98.2% amino acid identity with the Drosophila BR-C and Manduca BR-C in the N-terminal BTB domain; 96.0%, 90.7%, and 85.2% identity with the three zinc finger domains of the Drosophila Z1, Z2, and Z4 isoforms; and 96.3% and 98.1% identity with the two zinc finger domains of the Manduca Z2 and Z4 isoforms, respectively. Partial genomic sequencing (from the 3' region of the core sequence to the 3' region of the Z3 class zinc finger-coding sequence) of the BmBR-C gene showed that four exons coding the zinc finger domains are arranged the same as the BR-C gene in Drosophila. The amino...Continue Reading

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Citations

May 29, 2007·Development Genes and Evolution·Rebecca F Spokony, Linda L Restifo
Oct 26, 2006·BMC Developmental Biology·Zhong Zheng GuiByung Rae Jin
Jul 27, 2007·BMC Developmental Biology·Shiping LiuZhonghuai Xiang
Mar 1, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Md Saheb AliHideki Kawasaki
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May 18, 2007·Gene·Jie TangCong-Zhao Zhou
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Mar 15, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Arun BuaklinSirawut Klinbunga
Jul 13, 2014·Insect Biochemistry and Molecular Biology·Lin LingAnjiang Tan

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