Structure and expression of the human Na,K-ATPase beta 2-subunit gene

Gene
J AvilaP Martín-Vasallo

Abstract

We cloned and characterized the human Na,K-ATPase beta 2-subunit gene. The gene encompasses over 8 kb at chromosome 17 in the human genome and is composed of seven exons. Primer extension analysis identified a major transcription initiation site 529 bases upstream of the translation start site. The 5'-flanking region of the gene harbors a potential TATA sequence, located 94 bases upstream of the transcription initiation site and a number of potential promoter and regulatory elements, among them a Sp1 site, at position -120. A functional Sp1 site has also been found in the rat Na,K-ATPase beta 2-subunit gene (Kawakami, K., Watanabe, Y., Araki, M., Nagano, K., 1993). Sp1 binds to the adhesion molecule on glia regulatory element that functions as a positive transcription regulatory element in astrocytes. (J. Neurosci. Res. 35, 138-146). Putative AATAAA and TG sequences were found at positions 7018 and 7068, respectively. These signals delimit the origin of the the poly(A) tail and mark the end of the sequence that completes the 3'-UT downstream sequence of the human cDNA. An Alu repetitive sequence is located between positions 5961 and 6274. The gene is expressed as a single mRNA species, of 3.36 kb, which is present in cerebrum, ...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1990·The Journal of Cell Biology·S GloorM Schachner
Nov 25, 1990·Nucleic Acids Research·J P Magyar, M Schachner
Dec 1, 1989·Journal of Neurochemistry·T PaladinoJ W Gurd
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R J BrittenE H Davidson
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·B Sun, P M Salvaterra
Jan 3, 1994·Biochimica Et Biophysica Acta·N HernandoM Coca-Prados
Sep 13, 1996·The Journal of Biological Chemistry·N MalikR Levenson
Oct 1, 1996·The Journal of Clinical Investigation·J WangA A McDonough
Mar 1, 1997·Trends in Biochemical Sciences·K FrechT Werner

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Citations

Apr 12, 2002·The International Journal of Biochemistry & Cell Biology·Diego Alvarez de la RosaPablo Martín-Vasallo
Jun 21, 2013·The Journal of Endocrinology·Milan ObradovicEsma R Isenovic
Oct 22, 2002·Proceedings of the National Academy of Sciences of the United States of America·Joseph F HoffmanDouglas R Yingst
Apr 16, 2015·International Journal of Biometeorology·Rajib DebB Prakash
Feb 9, 2000·Genomics·P CousinP Shaw
Nov 9, 2006·The Journal of Biological Chemistry·Naoko FujimuraZbynek Kozmik
Nov 19, 2015·Frontiers in Cell and Developmental Biology·Zhiqin Li, Sigrid A Langhans

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