Abstract
To identify the VIP biosynthetic pathways, we have isolated the human VIP gene, using synthetic oligodeoxynucleotides. These specific hybridization probes were constructed according to the neuroblastoma VIP-cDNA sequence and contained up to 39 bases. The gene structure was deduced by direct chemical nucleotide sequencing. Six exons were thus far discovered; among them two short exons, one encoding VIP and the second encoding PHM-27 (a peptide having a N-terminal histidine and C-terminal methionine amide, closely related in sequence and activity to VIP). As a model system for VIP gene expression, we used a human buccal tumor producing elevated amounts of VIP. In these cells, a major transcript of the VIP-gene was identified as a long RNA containing intron sequences. The occurrence of elevated quantities of a high molecular weight, intron containing, gene transcript which is not processed directly into mature RNA suggests that VIP gene expression may be regulated at the RNA processing level.
References
Mar 21, 1977·Biochimica Et Biophysica Acta·I M Verma
Jun 15, 1977·Journal of Molecular Biology·P W RigbyP Berg
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M BodnerI Gozes
Apr 8, 1985·FEBS Letters·M NishizawaH Okamoto
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K E MayoR M Evans
Feb 14, 1968·Journal of Molecular Biology·J G Wetmur, N Davidson
Nov 19, 1983·Lancet·S R BloomJ M Polak
Mar 1, 1984·Peptides·S I Said
Jan 1, 1980·Methods in Enzymology·J FavaloroR Kamen
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Mar 25, 1980·Nucleic Acids Research·C AuffrayF Rougeon
Jul 15, 1982·Nature·S G AmaraR M Evans
Apr 30, 1984·Biochemical and Biophysical Research Communications·C GespachG Rosselin
Mar 1, 1984·Peptides·I GozesM Fridkin
Jan 1, 1984·Journal of Cellular Biochemistry·I GozesM Fridkin
Aug 11, 1983·Nature·N ItohH Okamoto
Oct 16, 1980·Nature·W AltenburgerH G Zachau
Jul 3, 1983·Nature·G I BellR C Najarian
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G Dalbadie-McFarlandJ H Richards
Jan 1, 1980·European Journal of Biochemistry·I GozesU Z Littauer
Citations
Jul 1, 1987·Somatic Cell and Molecular Genetics·I GozesT B Shows
Jan 1, 1993·Journal of Molecular Neuroscience : MN·I Gozes, D E Brenneman
Jan 1, 1989·Molecular Neurobiology·I Gozes, D E Brenneman
Apr 1, 1995·Molecular Neurobiology·J M MullerA C Meunier
Jan 1, 1993·Journal of Molecular Neuroscience : MN·I GozesH Westphal
Jan 1, 1989·Journal of Molecular Neuroscience : MN·I GozesY Koch
Jul 9, 2008·Journal of Molecular Neuroscience : MN·Illana Gozes
Jul 1, 1987·Brain Research·I GozesW H Rostène
Apr 1, 1990·Brain Research. Molecular Brain Research·E GiladiI Gozes
Feb 1, 1991·Brain Research. Molecular Brain Research·E D LampertiL Villa-Komaroff
Feb 14, 1994·Neuroscience Letters·I GozesW Rostene
Oct 1, 1992·Neuroscience·M DussaillantW Rostene
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T W MoodyI Gozes
Nov 3, 2004·Regulatory Peptides·Margalit Zusev, Illana Gozes
Jan 1, 1988·Annals of the New York Academy of Sciences·I Gozes
Jun 7, 2005·General and Comparative Endocrinology·Ronit S KulickMohamed E El Halawani
Aug 3, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anna Lucia TorneselloFranco Maria Buonaguro
Apr 1, 1987·Journal of Neurochemistry·I GozesY Shani
Oct 4, 2006·Physiological Reviews·Dirk RoostermanMartin Steinhoff
Jun 1, 2004·Pharmacological Reviews·Mario DelgadoDoina Ganea
Apr 1, 1986·Journal of Endocrinological Investigation·S I Said