Structure and expressions of two circadian clock genes, period and timeless in the commercial silkmoth, Bombyx mori

Journal of Insect Physiology
Sachio IwaiMakio Takeda

Abstract

We cloned two circadian clock genes period (Bmper) and timeless (Bmtim) from the commercial silkmoth, Bombyx mori. Sequence analysis revealed a high degree of conservation among insects for both genes. BmPER predicted from the DNA sequence is a polypeptide of 1, 113 amino acids with functional domains such as PAS, PAC, nuclear localization signal (NLS) and cytoplasmic localization domain (CLD). Deduced BmTIM consists of 997 amino acids with PER interaction site (PIS) as well as NLS and CLD. Southern blot analyses revealed that Bmper and Bmtim are single copy genes. Northern blot analysis demonstrated that Bmper and Bmtim are expressed both in the head and peripheral tissues. We also examined temporal profiles of Bmper and Bmtim expressions in the head, flight muscle, testis and antenna of adult males under LD12:12 and LD16:8 by Real-Time PCR assays. Our data show that photoperiod differentially affects the temporal expression patterns of Bmper and Bmtim. The mRNA expression of Bmper and Bmtim in the head had a phase lead under LD12:12 compared to that under LD16:8, whereas photoperiod did not affect expression patterns in peripheral tissues relative to light-on. Photoperiod affected not only the phase relationship but also the ...Continue Reading

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Citations

Aug 3, 2007·Genetical Research·Federica SandrelliRodolfo Costa
Oct 30, 2010·Journal of Insect Physiology·Vladimír Koštál
Sep 25, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Sachio IwaiMakio Takeda
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