Structure and filament dynamics of the pSK41 actin-like ParM protein: implications for plasmid DNA segregation.

The Journal of Biological Chemistry
David PoppMaria A Schumacher

Abstract

Type II plasmid partition systems utilize ParM NTPases in coordination with a centromere-binding protein called ParR to mediate accurate DNA segregation, a process critical for plasmid retention. The Staphylococcus aureus pSK41 plasmid is a medically important plasmid that confers resistance to multiple antibiotics, disinfectants, and antiseptics. In the first step of partition, the pSK41 ParR binds its DNA centromere to form a superhelical partition complex that recruits ParM, which then mediates plasmid separation. pSK41 ParM is homologous to R1 ParM, a known actin homologue, suggesting that it may also form filaments to drive partition. To gain insight into the partition function of ParM, we examined its ability to form filaments and determined the crystal structure of apoParM to 1.95 A. The structure shows that pSK41 ParM belongs to the actin/Hsp70 superfamily. Unexpectedly, however, pSK41 ParM shows the strongest structural homology to the archaeal actin-like protein Thermoplasma acidophilum Ta0583, rather than its functional homologue, R1 ParM. Consistent with this divergence, we find that regions shown to be involved in R1 ParM filament formation are not important in formation of pSK41 ParM polymers. These data are also ...Continue Reading

References

Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M R Webb
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Sep 6, 1990·Nature·K C HolmesW Kabsch
Jan 1, 1965·Methods of Biochemical Analysis·K C Holmes, D M Blow
Apr 26, 1983·Biochemistry·J A CooperT D Pollard
Apr 1, 1983·Journal of Biochemistry·E Nishida, H Sakai
Jul 8, 1982·Nature·E H EgelmanD J DeRosier
Feb 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·W Kabsch, K C Holmes
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·L D BelmontE H Egelman
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·T C Terwilliger, J Berendzen
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·C R KissingerD B Fogel
Aug 10, 2000·Molecular Microbiology·K GerdesR Bugge Jensen
Jul 28, 2001·Science·L R OtterbeinR Dominguez
Sep 7, 2001·Nature·F van den EntJ Löwe
Feb 13, 2002·Current Opinion in Structural Biology·Warren L DeLano
Aug 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Elena P SablinRobert J Fletterick
Dec 18, 2002·The EMBO Journal·Fusinita van den EntJan Löwe
Oct 12, 2004·Biophysical Journal·Yaodong ChenHarold P Erickson
Nov 6, 2004·Science·Ethan C GarnerR Dyche Mullins
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·E Krissinel, K Henrick
Mar 2, 2005·Plasmid·Barbara E Funnell
Mar 19, 2005·The EMBO Journal·Daniela BarillàFinbarr Hayes
Nov 16, 2005·Annual Review of Genetics·Gitte Ebersbach, Kenn Gerdes
Jan 18, 2006·Nature Reviews. Microbiology·Finbarr Hayes, Daniela Barillà
Feb 28, 2006·Journal of Molecular Biology·Annette RoebenAndreas Bracher
Dec 19, 2006·Biochemical and Biophysical Research Communications·David PoppYuichiro Maéda
Jun 15, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Harold P Erickson
Sep 18, 2007·Nature Structural & Molecular Biology·Albina OrlovaEdward H Egelman
Sep 28, 2007·The EMBO Journal·Jakob Møller-JensenJan Löwe
Nov 28, 2007·The Journal of Cell Biology·Christopher S Campbell, R Dyche Mullins
Dec 22, 2007·Nature·Maria A SchumacherNeville Firth
Jun 3, 2008·The Journal of Biological Chemistry·Mitsusada IwasaToshiro Oda

❮ Previous
Next ❯

Citations

Feb 19, 2013·Plasmid·Michael A LiuNeville Firth
Feb 23, 2011·The Journal of Biological Chemistry·Christopher R RiveraR Dyche Mullins
Apr 20, 2012·The Journal of Biological Chemistry·David PoppRobert C Robinson
Dec 4, 2012·The Journal of Biological Chemistry·Ertan OzyamakArash Komeili
Mar 20, 2012·Journal of Bacteriology·Alan I DermanJoe Pogliano
May 21, 2014·ELife·Fusinita van den EntJan Löwe
Aug 24, 2011·The Journal of Cell Biology·Bill Wickstead, Keith Gull
Jul 1, 2015·Proceedings of the National Academy of Sciences of the United States of America·Tatjana BraunEdward H Egelman
Feb 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Shimin JiangRobert C Robinson
Mar 7, 2013·Journal of Structural Biology·Pananghat GayathriJan Löwe
Apr 26, 2015·Plasmid·Christopher J Harmer, Ruth M Hall
Jan 23, 2018·Nature Reviews. Microbiology·James Wagstaff, Jan Löwe
Feb 15, 2018·Proceedings of the National Academy of Sciences of the United States of America·Gülsima D UsluerJustin M Kollman
Jun 17, 2016·PloS One·Anthony J BrzoskaNeville Firth
Mar 3, 2011·Molecular Microbiology·David Popp, Robert C Robinson
Jan 11, 2012·Cytoskeleton·David Popp, Robert C Robinson
Oct 20, 2012·Environmental Microbiology·Anna-Lena HencheSonja-Verena Albers
Jan 4, 2017·Communicative & Integrative Biology·Shimin JiangRobert C Robinson
Jul 11, 2020·Advanced Biosystems·Daniel HürtgenVictor Sourjik
Mar 20, 2015·Journal of Cell Science·Peter W GunningRobert C Robinson
Dec 15, 2018·Microbiology Spectrum·Neville FirthJoshua P Ramsay
Jun 25, 2015·Microbiology Spectrum·Jamie C Baxter, Barbara E Funnell
Jun 13, 2019·EcoSal Plus·Jean-Yves Bouet, Barbara E Funnell
Sep 11, 2013·Biochemistry·Ertan OzyamakArash Komeili
Feb 11, 2012·Current Biology : CB·Vitold E GalkinEdward H Egelman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.