Jun 15, 1976

Structure and fluctuation of a Streptomyces subtilisin inhibitor

Biochimica Et Biophysica Acta
M Nakanishi, M Tsuboi

Abstract

The kinetics of the hydrogen-deuterium exchange reaction in a subtilisin inhibitor from Streptomyces albogriseolus has been examined by infrared absorption measurement in aqueous solutions at various pH values and temperatures. In the analysis of each piece of kinetic data, it was assumed that the total 104 peptide hydrogen atoms are classified into three kinetic classes A, B1, and B2, and that the sizes of these classes are 72, 15, and 17, respectively at every pH and at every temperature examined. On the basis of the peak position determined for the amide II band in each stage of the exchange reaction, an approximate assignment was suggested of the A, B1 and B2 respectively to an unordered structure, a beta-structure,and an alpha-helical structure in the molecule. This assignment was supported by infrared absorption measurement of a film of this protein and by circular dichroic study of the solutions. On the basis of the temperature effect on the hydrogen-exchange rate constants and on the basis of ultraviolet absorption study in the higher temperature region (40 to 90 degrees C), a discussion has been made on the nature of the fluctuation of the molecular structure of this protein.

  • References3
  • Citations7

References

Mentioned in this Paper

Thermodynamics
Bacterial Proteins
Plasma Protein Binding Capacity
Calorimetry
Infrared Spectrophotometry
Protein Conformation
Spectrophotometry, Ultraviolet
Subtilisin 72
Circular Dichroism, Vibrational
Deuterons

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