Structure and function of a nitrifying biofilm as determined by in situ hybridization and the use of microelectrodes.

Applied and Environmental Microbiology
A SchrammK H Schleifer

Abstract

Microprofiles of O2 and NO3- were measured in nitrifying biofilms from the trickling filter of an aquaculture water recirculation system. By use of a newly developed biosensor for NO3-, it was possible to avoid conventional interference from other ions. Nitrification was restricted to a narrow zone of 50 microns on the very top of the film. In the same biofilms, the vertical distributions of members of the lithoautotrophic ammonia-oxidizing genus Nitrosomonas and of the nitrite-oxidizing genus Nitrobacter were investigated by applying fluorescence in situ hybridization of whole fixed cells with 16S rRNA-targeted oligonucleotide probes in combination with confocal laser-scanning microscopy. Ammonia oxidizers formed a dense layer of cell clusters in the upper part of the biofilm, whereas the nitrite oxidizers showed less-dense aggregates in close vicinity to the Nitrosomonas clusters. Both species were not restricted to the oxic zone of the biofilm but were also detected in substantially lower numbers in the anoxic layers and even occasionally at the bottom of the biofilm.

References

Jan 1, 1979·Annual Review of Microbiology·L W Belser
Feb 1, 1992·Applied and Environmental Microbiology·R I AmannD A Stahl
Oct 1, 1978·Applied and Environmental Microbiology·L W Belser, E L Schmidt
Oct 1, 1986·Applied and Environmental Microbiology·M Poth
Apr 1, 1987·Applied and Environmental Microbiology·A Abeliovich
Feb 1, 1993·Applied and Environmental Microbiology·D de BeerS P Ottengraf
Apr 1, 1996·Applied and Environmental Microbiology·L H LarsenS J Binnerup

❮ Previous
Next ❯

Citations

Sep 30, 2000·Applied and Environmental Microbiology·S E CowanJ D Keasling
Sep 7, 2001·Annual Review of Microbiology·H N Schulz, B B Jorgensen
Dec 9, 2003·Applied and Environmental Microbiology·Aurélie CébronJosette Garnier
Jul 8, 2005·Applied and Environmental Microbiology·Satoshi OkabeTsukasa Ito
Oct 20, 2005·Canadian Journal of Microbiology·Sharon Avrahami, Ralf Conrad
Jan 13, 2006·Bioprocess and Biosystems Engineering·Michael RodgersEdmond O'Reilly
Feb 12, 2008·Nature Reviews. Microbiology·Philip S Stewart, Michael J Franklin
Mar 21, 2009·Applied Microbiology and Biotechnology·Zhi-Wu Wang, Shulin Chen
Apr 16, 2015·Microbial Biotechnology·Micol BellucciThomas P Curtis
Dec 7, 2017·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Yoshiaki HasebeSatoshi Tsuneda
Oct 6, 1998·Applied and Environmental Microbiology·C M Santegoeds de Beer D
Apr 27, 2002·Applied and Environmental Microbiology·Bjarke B ChristensenSøren Molin
Mar 7, 2001·Applied and Environmental Microbiology·A GiesekeA Schramm
Nov 28, 2001·Applied and Environmental Microbiology·P C BurrellT A Hovanec
Feb 24, 2001·Environmental Microbiology·A SchrammR Amann
May 13, 2005·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Bulent MertogluIzzet Ozturk
Nov 15, 2000·FEMS Microbiology Reviews·R Amann, W Ludwig
Sep 9, 2006·FEMS Microbiology Ecology·Joke GeetsWilly Verstraete
Aug 18, 2005·Environmental Microbiology·Armin GiesekeDirk de Beer
Feb 6, 2007·Applied and Environmental Microbiology·Tina TreudeAntje Boetius
Jul 28, 2012·Water Environment Research : a Research Publication of the Water Environment Federation·Robert DelatollaDimitrios Berk
Feb 1, 2011·Biotechnology and Bioengineering·Ryan RenslowHaluk Beyenal
Oct 17, 2014·Environmental Technology·Marcin Spychała, Justyna Starzyk
Mar 17, 2015·Environmental Science and Pollution Research International·Chao WangBaoqing Shan
Dec 20, 2016·Measurement Science & Technology·Xiangmeng MaDarren A Lytle
Feb 17, 1999·FEMS Microbiology Reviews·M S JettenJ G Kuenen
Sep 9, 2005·Microbiology and Molecular Biology Reviews : MMBR·Jakob Pernthaler, Rudolf Amann
Aug 21, 2007·Biotechnology and Bioengineering·Daniel P SmithJay L Garland
Dec 7, 2005·FEMS Microbiology Ecology·Roeland GrommenWilly Verstraete
May 29, 2007·Applied and Environmental Microbiology·Tomonori KindaichiSatoshi Okabe

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.