PMID: 9431914Feb 12, 1998Paper

Structure and function of plasmid pColD157 of enterohemorrhagic Escherichia coli O157 and its distribution among strains from patients with diarrhea and hemolytic-uremic syndrome

Journal of Clinical Microbiology
C HofingerH Schmidt

Abstract

In this study, pColD157, a 6.7-kb colicinogenic plasmid of enterohemorrhagic Escherichia coli (EHEC) O157:H7 strain CL40cu, was characterized by restriction mapping and determination of its complete nucleotide sequence. The sequence consists of 6,675 bp and shows a high degree of similarity to the nucleotide sequence of colicinogenic plasmids pColD-CA23 and pColK. Seven potential genes were located on pColD157, three of which were closely related (>97.9%) to the colicin D structural gene and the corresponding immunity and lysis genes of plasmid pColD-CA23, and these were therefore designated cda, cdi, and cdl, respectively, using the reference extension -CL40 for differentiation. The adjacent 3' region is related to the origin of replication of pColD-CA23. In contrast, the remaining part of the plasmid harbors a cluster of genes, closely related to the mobilization genes of pColK, which is followed by a 0.3-kb stretch homologous to the pColK resolution function. These determinants were designated mbdA, mbdB, mbdC, and mbdD and cdr, respectively. Southern blot analysis was performed with a probe specific for the cda gene of pColD157 and two groups of EHEC O157:H7 isolates from patients with diarrhea or hemolytic-uremic syndrome ...Continue Reading

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Citations

May 11, 2005·Journal of Molecular Evolution·Milind ChavanMargaret A Riley
May 20, 2008·Applied and Environmental Microbiology·Kim ZiebellVictor Gannon
Sep 30, 2000·Applied and Environmental Microbiology·S YaronK R Matthews
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Eric CascalesDanièle Cavard
Mar 12, 2016·Bioscience, Biotechnology, and Biochemistry·Tetsuhiro Ogawa
Mar 5, 2004·Journal of Bacteriology·John E Wertz, Margaret A Riley
Feb 25, 2011·Journal of Clinical Microbiology·Ping WangJianguo Xu
Dec 22, 2004·Journal of Applied Microbiology·G P Schamberger, F Diez-Gonzalez
Mar 31, 2009·Applied and Environmental Microbiology·Marina SteeleVictor Gannon
Nov 9, 2002·Biochimie·Volkmar BraunKlaus Hantke

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