Structure and function of S-adenosylhomocysteine hydrolase

Cell Biochemistry and Biophysics
M A TurnerP L Howell

Abstract

In mammals, S-adenosylhomocysteine hydrolase (AdoHcyase) is the only known enzyme to catalyze the breakdown of S-adenosylhomocysteine (AdoHcy) to homocysteine and adenosine. AdoHcy is the product of all adenosylmethionine (AdoMet)-dependent biological transmethylations. These reactions have a wide range of products, and are common in all facets of biometabolism. As a product inhibitor, elevated levels of AdoHcy suppress AdoMet-dependent transmethylations. Thus, AdoHcyase is a regulator of biological transmethylation in general. The three-dimensional structure of AdoHcyase complexed with reduced nicotinamide adenine dinucleotide phosphate (NADH) and the inhibitor (1'R, 2'S, 3'R)-9-(2',3'-dihyroxycyclopenten-1-yl)adenine (DHCeA) was solved by a combination of the crystallographic direct methods program, SnB, to determine the selenium atom substructure and by treating the multiwavelength anomalous diffraction data as a special case of multiple isomorphous replacement. The enzyme architecture resembles that observed for NAD-dependent dehydrogenases, with the catalytic domain and the cofactor-binding domain each containing a modified Rossmann fold. The two domains form a deep active site cleft containing the cofactor and bound inhib...Continue Reading

Citations

Jun 24, 2009·Naunyn-Schmiedeberg's Archives of Pharmacology·Doris KloorHartmut Osswald
Dec 18, 2004·Plant Molecular Biology·William G Heim, John G Jelesko
Feb 19, 2004·Experimental Parasitology·Nathan B ParkerDaniel H Yin
Mar 16, 2012·Analytical Chemistry·Emmanuel S BurgosVern L Schramm
Oct 20, 2006·Organic Letters·Daniela Andrei, Stanislaw F Wnuk
Aug 3, 2005·The Journal of Experimental Medicine·Fabien BlanchetOreste Acuto
Apr 7, 2010·Journal of Experimental Botany·Jisen ShiRen-Hua Zheng
Feb 22, 2012·Acta Crystallographica. Section D, Biological Crystallography·Krzysztof BrzezinskiMariusz Jaskolski
Sep 19, 2002·Protein Science : a Publication of the Protein Society·Dmitri E Fomenko, Vadim N Gladyshev
Feb 3, 2007·Genome Biology·Christopher D ToscanoFrancesca Bosetti
Mar 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ivo BaricS Harvey Mudd
Feb 26, 2010·Nucleosides, Nucleotides & Nucleic Acids·Yeon Hee ParkLak Shin Jeong
Feb 26, 2010·Nucleosides, Nucleotides & Nucleic Acids·Qing-Shan LiRichard L Schowen
Feb 26, 2010·Nucleosides, Nucleotides & Nucleic Acids·Marco RadiChung K Chu
Oct 11, 2015·Journal of Pharmaceutical Sciences·Katharine Barbara SchowenMichael S Wolfe
Apr 17, 2012·Journal of Proteomics·Jonathan de Magalhães AndradeJoão Roberto Oliveira do Nascimento
Sep 26, 2008·Protein Science : a Publication of the Protein Society·Manchi C M ReddyJames C Sacchettini
Jul 19, 2011·Alcoholism, Clinical and Experimental Research·Tracy R Butler, Mark A Prendergast
Nov 11, 2005·Biochimica Et Biophysica Acta·Yolanda Pérez-PertejoRafael Balaña-Fouce
Jul 12, 2005·Molecular and Biochemical Parasitology·Masayuki NakanishiYukio Kitade
Nov 28, 2012·Biochemical and Biophysical Research Communications·Rosa Milagros CorralesLaurent Kremer
Mar 3, 2009·Biochemical and Biophysical Research Communications·Eun Young ParkHyun Kyu Song
Jun 15, 2007·FEMS Microbiology Letters·Vlasta CtrnáctáIvan Hrdý
Nov 12, 2016·Animal Reproduction Science·Mitra ArianmaneshKaïs H Al-Gubory
Nov 30, 2016·Aging Cell·Jian-Guo LiDomenico Praticò
Feb 24, 2017·International Journal of Molecular Sciences·Li ZhangKa Fai To
Apr 15, 2004·Bioorganic & Medicinal Chemistry Letters·Hea Ok KimLak Shin Jeong
Aug 18, 2009·Bioorganic & Medicinal Chemistry·Stanislaw F WnukDehua Pei

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antiparasitics

Antiparasitics are medications which are indicated for the treatment of parasitic diseases. Discover the latest research on antiparasitics here.