Structure and function of sawB, a gene involved in differentiation of Streptomyces ansochromogenes

Science in China. Series C, Life Sciences
L NieHuarong Tan

Abstract

A partial DNA library of Streptomyces ansochromogenes 7100 was constructed by using plasmid pl J702 as vector and white mutant W19 as recipient. About 3 000 clones were obtained, two of which gave rise to the grey phenotype as wild type 7100. The plasmids were isolated from two transformants. The result indicated that the 5.2 kb and 5.8 kb DNA fragments were inserted into plJ702. The resulting recombinant plasmids were designated as pNL-1 and pNL-2 respectively. The 1.25 kb Pstl I -Apa I DNA fragment from pNL-1 was recognized as its complementarity to W19 strain. The nucleotide sequence of the 3.0 kb Pst I DNA fragment including 1.25 kb was determined and analyzed. The result indicated that this DNA fragment contains one complete open reading frame (ORF1) which encodes a protein with 295 amino acid residues, and this gene was designated as sawB. The deduced protein has 81% amino acid identities in comparison with that encoded by whiH in Streptomyces coelicolor. The function of sawB gene was studied by using strategy of gene disruption, and the resulting sawB mutant failed to form spores and produced loosely coiled aerial hyphal. The result showed that sawB is closely related to hyphal coiling and sporulation in S. ansochromogen...Continue Reading

References

Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B K LeskiwK F Chater
Apr 1, 1990·Antimicrobial Agents and Chemotherapy·R F HectorD Pappagianis
Nov 1, 1989·Trends in Genetics : TIG·K F Chater
Dec 1, 1987·Journal of Bacteriology·C Mendez, K F Chater
Jan 1, 1993·Annual Review of Microbiology·K F Chater

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