PMID: 1569007May 1, 1992Paper

Structure and function of the uhp genes for the sugar phosphate transport system in Escherichia coli and Salmonella typhimurium

Journal of Bacteriology
M D IslandR J Kadner


Expression of the Escherichia coli sugar phosphate transport system, encoded by the uhpT gene, is regulated by external glucose 6-phosphate through the action of three linked regulatory genes, uhpABC. The nucleotide sequence of the uhp region cloned from Salmonella typhimurium was determined. The deduced Uhp polypeptide sequences from the two organisms are highly related. Comparison with the corrected sequence from E. coli revealed that the four uhp genes are closely spaced, with minimal intergenic distances, and that uhpC is nearly identical in length to uhpT, both of which have substantial sequence relatedness along their entire lengths. To facilitate analysis of uhp gene function, we isolated insertions of a kanamycin resistance (Km) cassette throughout the uhp region. In-frame deletions that removed almost the entire coding region of individual or multiple uhp genes were generated by use of restriction sites at the ends of the Km cassette. The phenotypes of the Km insertions and the in-frame deletions confirmed that all three regulatory genes are required for Uhp function. Whereas the deletion of uhpA completely abolished the expression of a uhpT-lacZ reporter gene, the deletion of uhpB or uhpC resulted in a partially eleva...Continue Reading


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Related Concepts

UhpT protein, E coli
UhpB protein, Salmonella typhimurium
UhpC protein, bacteria
UhpC protein, E coli
UhpC protein, Salmonella typhimurium
Bacterial Proteins
Carrier Proteins
Genome Mapping
DNA Mutational Analysis
Alkalescens-Dispar Group

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