PMID: 1569007May 1, 1992Paper

Structure and function of the uhp genes for the sugar phosphate transport system in Escherichia coli and Salmonella typhimurium

Journal of Bacteriology
M D IslandR J Kadner

Abstract

Expression of the Escherichia coli sugar phosphate transport system, encoded by the uhpT gene, is regulated by external glucose 6-phosphate through the action of three linked regulatory genes, uhpABC. The nucleotide sequence of the uhp region cloned from Salmonella typhimurium was determined. The deduced Uhp polypeptide sequences from the two organisms are highly related. Comparison with the corrected sequence from E. coli revealed that the four uhp genes are closely spaced, with minimal intergenic distances, and that uhpC is nearly identical in length to uhpT, both of which have substantial sequence relatedness along their entire lengths. To facilitate analysis of uhp gene function, we isolated insertions of a kanamycin resistance (Km) cassette throughout the uhp region. In-frame deletions that removed almost the entire coding region of individual or multiple uhp genes were generated by use of restriction sites at the ends of the Km cassette. The phenotypes of the Km insertions and the in-frame deletions confirmed that all three regulatory genes are required for Uhp function. Whereas the deletion of uhpA completely abolished the expression of a uhpT-lacZ reporter gene, the deletion of uhpB or uhpC resulted in a partially eleva...Continue Reading

Citations

Feb 15, 1994·FEMS Microbiology Letters·Valérie Dumay, Martine Crasnier
Dec 12, 2001·Microbiology·Daniël T VerhammeKlaas J Hellingwerf
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·M GoetzWerner Goebel
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·H Ekkehard Neuhaus, M. J. Emes
Nov 20, 2012·Nature·Alline R PachecoVanessa Sperandio
May 22, 2019·Journal of Bacteriology·Tristan CerisyAndrew C Tolonen
May 23, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fuping SongChristina Nielsen-LeRoux
Oct 22, 1998·FEBS Letters·P MarcolongoV Sorrentino
Dec 5, 1998·The Journal of Biological Chemistry·M C Fann, P C Maloney
May 13, 1999·The Journal of Biological Chemistry·C J PanJ Y Chou
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Isabel Chico-CaleroEuropean Listeria Genome Consortium
Mar 26, 2003·European Journal of Biochemistry·Christian SchwöppeH Ekkehard Neuhaus
May 3, 2018·Frontiers in Chemistry·Anna Rita CappelloVincenza Dolce
Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Mar 11, 2000·European Journal of Biochemistry·G van de WerveA Berteloot
Jul 27, 2000·Journal of Bacteriology·Q Chen, R J Kadner
Jun 26, 2007·Journal of Bacteriology·Elizabeth A HussaKaren L Visick
Nov 2, 2004·Journal of Bacteriology·Alberto Tierrez, Francisco García-del Portillo
Apr 28, 2001·Journal of Bacteriology·J S Wright, R J Kadner
Nov 17, 2020·Frontiers in Microbiology·Vincent CattoirFrançois Guérin
Jun 15, 1999·Journal of Bacteriology·B González-Flecha, B Demple
Jul 1, 1997·Protein Expression and Purification·E TamaiP C Maloney

Related Concepts

UhpT protein, E coli
UhpB protein, Salmonella typhimurium
UhpC protein, bacteria
UhpC protein, E coli
UhpC protein, Salmonella typhimurium
Bacterial Proteins
Carrier Proteins
Genome Mapping
DNA Mutational Analysis
Alkalescens-Dispar Group

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Laryngeal Neoplasms

Laryngeal Neoplasms occur in the Larynx and are typically associated with smoking and alcohol consumption. Discover the latest research on Laryngeal Neoplasms here.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.