PMID: 16521573Mar 9, 2006Paper

Structure and functional properties of temperature-sensitive derivatives of immobilized proteins

Prikladnaia biokhimiia i mikrobiologiia
L I ValuevI M Shanazarova

Abstract

Polydiethylacrylamides (degree of polymerization, 13-470) containing a terminal carboxyl group were obtained by the method of radical polymerization of N,N-diethylacrylamide in the presence of mercaptoacetic acid. In the presence of 1-ethyl-(3,3-dimethylaminopropyl)-carbodiimide, these polymers reacted with ovomucoid to produce its polymeric derivatives. The values of the lower critical mixing temperature of these derivatives and the inhibitory activities of immobilized ovomucoid were determined by the length and amount of polydiethylacrylamide macromolecules bound to the molecule of ovomucoid.

References

Jul 1, 1966·Archives of Biochemistry and Biophysics·B F ErlangerA G Cooper
Jun 14, 2002·Trends in Biotechnology·Byeongmoon Jeong, Anna Gutowska
Feb 1, 1949·Journal of Colloid Science·O KRATKY, G POROD

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Citations

Jun 15, 2012·Journal of Food Science·Yuanyuan ShanYongguo Jin

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