PMID: 2492099Jan 1, 1989Paper

Structure and heterogeneity of the oligosaccharides from the lipopolysaccharides of a pyocin-resistant Neisseria gonorrhoeae

Proceedings of the National Academy of Sciences of the United States of America
B W GibsonJ McLeod Griffiss

Abstract

The compositions and partial structures of the oligosaccharides from the lipopolysaccharides (LPS) of a pyocin-resistant Neisseria gonorrhoeae (strain JW31R) have been determined by liquid secondary ion mass spectrometry (LSIMS), tandem mass spectrometry, and methylation analysis. Four major structures were identified with Mr 2123, 2000, 1961, and 1838, as well as seven species of lower abundance of Mr 1758-1272. The largest of the major oligosaccharides (Mr, 2122) consists of 3-deoxymanno-2-ketooctulosonic acid (KDO)-Hep2GalNAcGlcNAcGal4Glc2 (Hep, heptose) and phosphoethanolamine (PEA). The smaller oligosaccharides are truncated versions of this larger oligosaccharide. The oligosaccharides consist of a common triantennary structure containing KDO at the reducing terminus attached to a heptose disaccharide. A hexose (Hex)2-3 branch is attached to the heptose linked directly to KDO and a GalNAc-Hex3, GlcNAc, and PEA are separately attached to the second heptose. These oligosaccharides are the first structures to be determined for a gonococcal LPS and should further our understanding of the structural and antigenic diversity of these glycolipids.

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Citations

Jan 1, 1991·Transactions of the Royal Society of Tropical Medicine and Hygiene·J M GriffissJ J Kim
Dec 15, 1991·FEMS Microbiology Letters·R Sandlin, D C Stein
Mar 10, 2009·Journal of Bacteriology·Ellen T O'ConnorDaniel C Stein
Feb 24, 2001·Journal of Bacteriology·D ArkingD C Stein
Nov 12, 2002·Journal of Bacteriology·Andrzej Piekarowicz, Daniel C Stein
Feb 9, 2012·Bioscience, Biotechnology, and Biochemistry·Ryohei YamasakiIchie Aoto
Nov 1, 1990·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·T F Meyer
Mar 29, 2000·The Journal of Biological Chemistry·J McLeod GriffissW Zollinger
Nov 1, 1990·Biomedical & Environmental Mass Spectrometry·N J PhillipsJ M Griffiss
Jan 1, 1996·Critical Reviews in Microbiology·A PrestonM A Apicella
Mar 7, 2002·International Reviews of Immunology·S GulatiP A Rice
Sep 1, 1989·Infection and Immunity·E F Petricoin, D C Stein
May 1, 1991·Journal of Bacteriology·R E MandrellR Yamasaki
Dec 1, 1995·Journal of Bacteriology·R J DanaherD C Stein

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