Structure and intracellular localization of mouse ADP-ribosylation factors type 1 to type 6 (ARF1-ARF6)

Journal of Biochemistry
M HosakaK Nakayama

Abstract

ADP-ribosylation factors (ARFs) are a family of small GTP-binding proteins that are proposed to be involved in the formation of coated transport vesicles. Although six ARF sequences have been reported in mammals to date, it has been unclear how many ARF members are present in a single organism. In this study, we provide the first direct evidence by cDNA cloning for the presence of all six ARF members in mouse. These proteins are highly conserved across mammalian species and Northern blot analysis revealed that mRNAs for all the members were expressed ubiquitously. Transfection of cells with epitope-tagged ARFs revealed that ARFs 1-3 displayed a perinuclear Golgi localization, while ARFs 4-6 appeared to be widely dispersed throughout the cytoplasm. These results suggest that although all the ARF proteins play fundamental and critical roles in cellular function, they are involved in different vesicular transport processes.

Citations

Jan 7, 2000·Biochemical and Biophysical Research Communications·R TakeyaH Sumimoto
Aug 1, 2002·Life Sciences·Masa-aki HattoriNoboru Fujihara
Jun 7, 2003·Advances in Enzyme Regulation·Yasunori KanahoMasakazu Yamazaki
Jan 18, 2011·The Journal of Biological Chemistry·Zhiqiu ManHye-Won Shin
Jan 19, 1999·Annual Review of Cell and Developmental Biology·P GunningE Hardeman
Apr 23, 1999·Journal of Basic and Clinical Physiology and Pharmacology·M A Rizzo, G Romero
Oct 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Tomoo ShibaSoichi Wakatsuki
May 23, 1998·The Journal of Cell Biology·M PassreiterW W Just
Jul 28, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Senye TakahashiKazuhisa Nakayama
Aug 1, 2006·Biochemical and Biophysical Research Communications·Hiroyuki TakatsuHiroshi Ohno
Mar 6, 2010·Congenital Anomalies·Toshiaki WatanabeToru Fukui
Dec 20, 2003·American Journal of Physiology. Cell Physiology·Jaafar El-AnnanVladimir Marshansky
Aug 16, 2005·The Journal of Biological Chemistry·Dorothee LayWilhelm W Just
Jun 12, 2003·The Journal of Biological Chemistry·Rory MitchellMelanie S Johnson
Dec 1, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Victoria M ViradorVincent J Hearing
Dec 4, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yawei LiRichard A Kahn
Sep 14, 2012·Cell Structure and Function·Yumika KondoHye-Won Shin
Jul 16, 2003·The Journal of Biological Chemistry·Jun MatsukawaTetsuro Urushidani
Sep 1, 2005·Journal of Cell Science·Hye-Won ShinKazuhisa Nakayama
Jul 31, 2008·Journal of Cell Science·Selma Y DejgaardJohn F Presley
May 28, 2003·Biochemistry·Guangyu ZhuXuejun C Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.