PMID: 8155881Feb 1, 1994Paper

Structure and light-regulated expression of the gsa gene encoding the chlorophyll biosynthetic enzyme, glutamate 1-semialdehyde aminotransferase, in Chlamydomonas reinhardtii

Plant Molecular Biology
G L Matters, S I Beale

Abstract

The gsa gene, which encodes glutamate 1-semialdehyde (GSA) aminotransferase (GSAT), an enzyme in the chlorophyll and heme biosynthetic pathway, has been cloned from Chlamydomonas reinhardtii by complementation of an Escherichia coli hemL mutant. The deduced C. reinhardtii GSAT amino acid sequence has a high degree of similarity to GSAT sequences from barley, tobacco, soybean and various prokaryotic sources. In vitro enzyme activity assays from E. coli transformed with the C. reinhardtii GSAT cDNA showed that higher levels of GSAT activity are associated with the expression of the cDNA insert. Analysis of changes in mRNA levels in light:dark synchronized C. reinhardtii cultures was done by northern blotting. The level of GSAT mRNA nearly doubled during the first 0.5 h in the light and increased over 26-fold after 2 h in the light. This increase is comparable to previously reported increases in GSAT activity in dark-grown cultures transferred to the light, and is the first report of induction by light of a gene encoding an ALA biosynthetic enzyme in plant or algal cells. The accumulation of GSAT mRNA follows the pattern of chlorophyll accumulation and the pattern of chlorophyll a/b-binding protein (cabII-1) mRNA accumulation in t...Continue Reading

References

Nov 1, 1991·Journal of Photochemistry and Photobiology. B, Biology·F JasperU Johanningmeier
May 1, 1990·Molecular & General Genetics : MGG·J A Schloss
May 1, 1990·The Plant Cell·P M GilmartinN H Chua
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B Grimm
Apr 1, 1989·The British Journal of Oral & Maxillofacial Surgery·M Harris
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G GiulianoA R Cashmore
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S P MayfieldJ D Rochaix
Sep 1, 1985·Molecular and Cellular Biology·C D SilflowL P Ranum
Mar 1, 1984·Molecular and Cellular Biology·J A SchlossJ L Rosenbaum
Jul 1, 1993·The Plant Cell·S WaffenschmidtU W Goodenough

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Citations

Jun 1, 1995·Photosynthesis Research·Y J Avissar, P A Moberg
Aug 21, 1995·Molecular & General Genetics : MGG·C HungererD Jahn
Sep 7, 2005·Photosynthesis Research·Zinaida VasileuskayaChristoph F Beck
Oct 29, 2002·Biochimica Et Biophysica Acta·Felix Bohne, Hartmut Linden
Mar 22, 2000·Plant Science : an International Journal of Experimental Plant Biology·G Drazic, M Bogdanovic
Apr 15, 1996·European Journal of Biochemistry·S Reinbothe, C Reinbothe
Apr 10, 2003·Eukaryotic Cell·Pushpa KathirCarolyn D Silflow
Dec 13, 2005·Eukaryotic Cell·Ariane AtteiaSamuel I Beale
Dec 1, 2004·Annual Review of Genetics·Arthur R GrossmanChung Soon Im
Mar 31, 1995·The Journal of Biological Chemistry·J M FrustaciM R O'Brian
Apr 1, 2007·EcoSal Plus·Samuel I Beale

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