PMID: 2114862Jan 1, 1990Paper

Structure and mutation of a gene encoding a Mr 33,000 phycocyanin-associated linker polypeptide

Archives of Microbiology
R de LorimierS E Stevens

Abstract

The gene encoding a phycocyanin-associated linker polypeptide of Mr 33,000 from the cyanobacterium Synechococcus sp. PCC 7002 was found to be located adjacent and 3' to the genes encoding the alpha and beta subunits of phycocyanin. The identity of this gene, designated cpcC, was proven by matching the amino-terminal sequence of the authentic polypeptide with that predicted by the nucleotide sequence. A cpcC mutant strain of this cyanobacterium was constructed. The effect of the mutation was to prevent assembly of half the total phycocyanin into phycobilisomes. By electron microscopy, phycobilisomes from this mutant were shown to contain rod substructures composed of a single disc of hexameric phycocyanin, as opposed to two discs in the wild type. It was concluded that the Mr 33,000 linker polypeptide is required for attachment of the core-distal phycocyanin hexamer to the core-proximal one. Using absorption spectra of the wild type, CpcC-, and phycocyanin-less phycobilisomes, the in situ absorbances expected for specific phycocyanin-linker complexes were calculated. These data confirm earlier findings on isolated complexes regarding the influence of linkers on the spectroscopic properties of phycocyanin.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 9, 1990·Biochimica Et Biophysica Acta·R de LorimierS E Stevens
Jan 1, 1985·Annual Review of Biophysics and Biophysical Chemistry·A N Glazer
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R de LorimierS E Stevens
Jun 1, 1983·Hoppe-Seyler's Zeitschrift für physiologische Chemie·P FüglistallerH Zuber
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Jun 1, 1980·The Journal of Cell Biology·R C WilliamsA N Glazer
Jan 1, 1982·Annual Review of Microbiology·A N Glazer

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Citations

Oct 2, 2013·Photosynthesis Research·Mai Watanabe, Masahiko Ikeuchi
Jul 25, 1995·Nucleic Acids Research·A D BaxevanisD Landsman
Mar 1, 1992·Plant Physiology·R M de LorimierS E Stevens
May 1, 1992·European Journal of Biochemistry·M GlauserH Zuber
Aug 15, 2003·Nature·B PalenikJ Waterbury
Jul 28, 2020·Photosynthesis Research·Nathan SoulierDonald A Bryant
Sep 6, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Pasqual LiauwGenji Kurisu
Mar 2, 1999·Journal of Structural Biology·R MacColl

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